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果蝇RNA聚合酶II延伸因子DmS-II与小鼠S-II具有同源性，且与酵母PPR2具有序列相似性。

Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2.

作者信息

Marshall T K, Guo H, Price D H

机构信息

Department of Biochemistry, University of Iowa, Iowa City 52242.

出版信息

Nucleic Acids Res. 1990 Nov 11;18(21):6293-8. doi: 10.1093/nar/18.21.6293.

DOI:10.1093/nar/18.21.6293
PMID:2243775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC332494/
Abstract

DmSII is a Drosophila RNA polymerase II elongation factor which suppresses pausing by RNA polymerase II at specific sites on double stranded templates. Using antibodies produced against the purified protein, a Drosophila cDNA expression library was screened and a cDNA was isolated which encoded a portion of DmSII. When this cDNA was used to probe Kc cell mRNA the predominant species was found to be 1.4 kb in length. The original cDNA was used to screen a Drosophila Kc cell cDNA library resulting in the isolation of a 1.4 kb cDNA which was then sequenced. The deduced protein sequence for DmSII exhibited high similarity to mouse SII protein sequence. In addition, significant sequence similarity was found with the protein encoded by the yeast gene PPR2, which is involved in regulation of URA4 gene expression. The comparison of amino acid sequences suggests that DmSII is comprised of two domains homologous to mouse SII separated by a flexible, serine rich region of low homology. The shorter yeast protein has sequence similarity only to the carboxy terminal domain.

摘要

DmSII是一种果蝇RNA聚合酶II延伸因子，它可抑制RNA聚合酶II在双链模板上特定位点的停顿。利用针对纯化蛋白产生的抗体，对果蝇cDNA表达文库进行筛选，分离出一个编码DmSII一部分的cDNA。当用该cDNA探测Kc细胞mRNA时，发现主要种类的长度为1.4 kb。原始cDNA用于筛选果蝇Kc细胞cDNA文库，从而分离出一个1.4 kb的cDNA，然后对其进行测序。推导的DmSII蛋白序列与小鼠SII蛋白序列具有高度相似性。此外，还发现与酵母基因PPR2编码的蛋白有显著的序列相似性，PPR2参与URA4基因表达的调控。氨基酸序列比较表明，DmSII由与小鼠SII同源的两个结构域组成，中间被一个低同源性的富含丝氨酸的柔性区域隔开。较短的酵母蛋白仅与羧基末端结构域有序列相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98d8/332494/3f15129900ce/nar00205-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98d8/332494/3f15129900ce/nar00205-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98d8/332494/3f15129900ce/nar00205-0108-a.jpg

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本文引用的文献

1
Difference in phosphorylation of two factors stimulating RNA polymerase II of Ehrlich ascites tumor cells.刺激艾氏腹水瘤细胞RNA聚合酶II的两种因子磷酸化的差异。
Biochemistry. 1981 Apr 14;20(8):2286-92. doi: 10.1021/bi00511a033.
2
Interaction of a new polypeptide with yeast RNA polymerase B.一种新多肽与酵母RNA聚合酶B的相互作用。
J Biol Chem. 1980 Jan 10;255(1):12-5.
3
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
EMBO J. 1996 Oct 15;15(20):5557-66.
4
Genes encoding isoforms of transcription elongation factor TFIIS in Xenopus and the use of multiple unusual RNA processing signals.非洲爪蟾中转录延伸因子TFIIS同工型的编码基因及多种异常RNA加工信号的应用。
Nucleic Acids Res. 1996 Sep 15;24(18):3514-21. doi: 10.1093/nar/24.18.3514.
5
Synthesis of reinitiated transcripts by mammalian RNA polymerase II is controlled by elongation factor SII.哺乳动物RNA聚合酶II重新起始转录本的合成受延伸因子SII的控制。
EMBO J. 1993 Dec;12(12):4677-84. doi: 10.1002/j.1460-2075.1993.tb06156.x.
6
The sequence, and its evolutionary implications, of a Thermococcus celer protein associated with transcription.与转录相关的嗜热栖热菌蛋白质的序列及其进化意义。
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3854-6. doi: 10.1073/pnas.91.9.3854.
7
Self-splicing group I introns in eukaryotic viruses.真核病毒中的自我剪接I类内含子。
Nucleic Acids Res. 1994 Jul 11;22(13):2532-7. doi: 10.1093/nar/22.13.2532.
8
Identification of a decay in transcription potential that results in elongation factor dependence of RNA polymerase II.鉴定转录潜能的衰减，该衰减导致RNA聚合酶II对延伸因子的依赖性。
J Biol Chem. 1995 May 12;270(19):11238-44. doi: 10.1074/jbc.270.19.11238.
9
RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme.RNA complex.大肠杆菌依赖DNA的RNA聚合酶在二元酶-RNA复合物中的RNA切割和链延伸。
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3784-8. doi: 10.1073/pnas.91.9.3784.
10
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Nucleic Acids Res. 1991 Jan 25;19(2):413-21. doi: 10.1093/nar/19.2.413.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
4
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J Biol Chem. 1984 Jan 10;259(1):608-11.
5
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EMBO J. 1983;2(11):2071-3. doi: 10.1002/j.1460-2075.1983.tb01702.x.
6
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J Biol Chem. 1985 May 10;260(9):5739-44.
7
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J Biol Chem. 1987 Mar 5;262(7):3331-7.
9
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