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RNA聚合酶II通过一种序列特异性DNA结合蛋白进行的延伸因子SII依赖性转录。

Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.

作者信息

Reines D, Mote J

机构信息

Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.

出版信息

Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1917-21. doi: 10.1073/pnas.90.5.1917.

DOI:10.1073/pnas.90.5.1917
PMID:8446609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC45991/
Abstract

In eukaryotes the genetic material is contained within a coiled, protein-coated structure known as chromatin. RNA polymerases must recognize specific nucleoprotein assemblies and maintain contact with the underlying DNA duplex for many thousands of base pairs. Template-bound lac operon repressor from Escherichia coli arrests RNA polymerase II in vitro and in vivo [Kuhn, A., Bartsch, I. & Grummt, I. (1990) Nature (London) 344, 559-562; Deuschele, U., Hipskind, R. A. & Bujard, H. (1990) Science 248, 480-483]. We show that in a reconstituted transcription system, elongation factor SII enables RNA polymerase II to proceed through this blockage at high efficiency. lac repressor-arrested elongation complexes display an SII-activated transcript cleavage reaction, an activity associated with transcriptional read-through of a previously characterized region of bent DNA. This demonstrates factor-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein. Nascent transcript cleavage may be a general mechanism by which RNA polymerase II can bypass many transcriptional impediments.

摘要

在真核生物中，遗传物质包含在一种被称为染色质的盘绕的、蛋白质包裹的结构中。RNA聚合酶必须识别特定的核蛋白组装体，并与潜在的DNA双链保持数千个碱基对的接触。来自大肠杆菌的模板结合型乳糖操纵子阻遏物在体外和体内都会阻止RNA聚合酶II [库恩，A.，巴尔施，I. & 格鲁姆特，I.（1990年）《自然》（伦敦）344卷，559 - 562页；多伊舍尔，U.，希普斯金德，R. A. & 布亚德，H.（1990年）《科学》248卷，480 - 483页]。我们表明，在一个重组转录系统中，延伸因子SII能使RNA聚合酶II高效地通过这种阻碍。乳糖阻遏物阻止的延伸复合物显示出SII激活的转录物切割反应，这种活性与先前表征的弯曲DNA区域的转录通读相关。这证明了RNA聚合酶II通过序列特异性DNA结合蛋白进行因子依赖性转录。新生转录物切割可能是RNA聚合酶II绕过许多转录阻碍的一种普遍机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/bfcef97864f1/pnas01464-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/d64518ea2659/pnas01464-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/d3ad30fb9f04/pnas01464-0294-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/08151019d0a7/pnas01464-0294-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/bfcef97864f1/pnas01464-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/d64518ea2659/pnas01464-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/d3ad30fb9f04/pnas01464-0294-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/08151019d0a7/pnas01464-0294-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/45991/bfcef97864f1/pnas01464-0295-a.jpg

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Stimulatory proteins of RNA polymerase II from Ehrlich ascites tumor cells.艾氏腹水瘤细胞RNA聚合酶II的刺激蛋白
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Immunoglobulin gene transcription is activated by downstream sequence elements.免疫球蛋白基因转录由下游序列元件激活。
关键时刻——RNA聚合酶与DNA结合蛋白障碍
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