de Jong E B, Horsten B P, Goldschmidt H M
J Chromatogr. 1983 Nov 25;279:563-72. doi: 10.1016/s0021-9673(01)93658-1.
A method is described for the simultaneous determination of nine urinary acidic and alcoholic catecholamine metabolites and urinary 5-hydroxyindolacetic acid. Incubation of a urine sample in the presence of ascorbic acid, glucuronidase and acylase and subsequent extraction with ethyl acetate precedes derivatization to trimethylsilyl compounds, capillary gas chromatographic separation and flame-ionization detection. The automated dual injection procedure and the analytical characteristics of the proposed method are reported in detail. Special attention is paid to problems that occur in analysis on a routine basis.
本文描述了一种同时测定九种尿酸性和醇性儿茶酚胺代谢物及尿5-羟吲哚乙酸的方法。尿样在抗坏血酸、葡萄糖醛酸酶和酰基酶存在下孵育，随后用乙酸乙酯萃取，再衍生化为三甲基硅烷基化合物，进行毛细管气相色谱分离和火焰离子化检测。详细报道了自动双进样程序及所提方法的分析特性。对常规分析中出现的问题给予了特别关注。
