气相色谱法同时测定尿液中的甲苯和二甲苯代谢物
Simultaneous determination of toluene and xylene metabolites in urine by gas chromatography.
作者信息
Caperos J R, Fernández J G
出版信息
Br J Ind Med. 1977 Aug;34(3):229-33. doi: 10.1136/oem.34.3.229.
Abstract
A gas chromatographic method for simultaneous determination of hippuric and o-, m-, and p-methylhippuric acids (metabolites of toluene and xylene) in urine is described. The analytical procedure is based on the extraction of the aromatic metabolites with ethyl acetate containing the internal standard and on a methylation with 3-methyl-1-p-tolyltriazene. With this method, which does not require much time and handling, the different acids can be satisfactorily determined with high sensitivity and specificity. A statistical study shows a good reproducibility for the determination of hippuric and o-, m-, and p-methylhippuric acids. The coefficient of variation for 10 determinations in all cases was less than 5%.
摘要
本文描述了一种气相色谱法，用于同时测定尿液中马尿酸以及邻、间、对甲基马尿酸（甲苯和二甲苯的代谢产物）。分析程序基于用含有内标的乙酸乙酯萃取芳香族代谢产物，并基于用3-甲基-1-对甲苯基三氮烯进行甲基化反应。采用这种方法，无需太多时间和操作，就能以高灵敏度和特异性令人满意地测定不同的酸。一项统计研究表明，测定马尿酸以及邻、间、对甲基马尿酸具有良好的重现性。在所有情况下，10次测定的变异系数均小于5%。
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Simultaneous determination of toluene and xylene metabolites in urine by gas chromatography.气相色谱法同时测定尿液中的甲苯和二甲苯代谢物
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Measurement of urinary hippuric and m-methylhippuric acids by gas chromatography.气相色谱法测定尿中马尿酸和间甲基马尿酸
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