Greenstein J L, Kappler J, Marrack P, Burakoff S J
J Exp Med. 1984 Apr 1;159(4):1213-24. doi: 10.1084/jem.159.4.1213.
The expression of T4/T8 surface markers on human T cells and of L3T4/Lyt-2 on murine T cells has lead to the association of these surface markers with recognition of either class II or class I major histocompatibility complex (MHC) antigens. It has been suggested that these T cell surface antigens interact with MHC antigens. We have examined the role of L3T4 in the recognition of Dd by the T cell hybridoma, 3DT52.5. This T cell hybridoma was found to be specific for the N/Cl domain of Dd. The recognition of a class I antigen by an Lyt-2-, L3T4+ T cell hybridoma allowed the separate evaluation of interactions between L3T4/Ia and the T cell antigen receptor, Dd. Recognition by this hybridoma resulted in the production of interleukin 2 (IL-2) and cytolytic activity. Antibody blocking experiments have demonstrated that L3T4 was involved in triggering the effector function of 3DT52.5 only on Ia+ stimulator or target cells. We have demonstrated that an L3T4+, Dd-specific T cell hybridoma, 3DT52.5, uses the L3T4 molecule to directly interact with nonpolymorphic Ia determinants.
人类T细胞上T4/T8表面标志物以及鼠类T细胞上L3T4/Lyt - 2的表达,使得这些表面标志物与对II类或I类主要组织相容性复合体(MHC)抗原的识别相关联。有人提出这些T细胞表面抗原与MHC抗原相互作用。我们研究了L3T4在T细胞杂交瘤3DT52.5识别Dd中的作用。发现该T细胞杂交瘤对Dd的N/Cl结构域具有特异性。Lyt - 2阴性、L3T4阳性的T细胞杂交瘤对I类抗原的识别,使得能够分别评估L3T4/Ia与T细胞抗原受体Dd之间的相互作用。该杂交瘤的识别导致白细胞介素2(IL - 2)的产生和细胞溶解活性。抗体阻断实验表明,L3T4仅在Ia阳性刺激细胞或靶细胞上参与触发3DT52.5的效应功能。我们已经证明,L3T4阳性、Dd特异性的T细胞杂交瘤3DT52.5利用L3T4分子直接与非多态性Ia决定簇相互作用。