Johannessen J N, Watkins L R, Mayer D J
J Neurosci. 1984 Mar;4(3):757-66. doi: 10.1523/JNEUROSCI.04-03-00757.1984.
Cells within the ventral medulla whose axons descend to the spinal dorsal horn via the dorsolateral funiculus (DLF) play an important role in pain modulation. Previous evidence suggests that this pathway is primarily serotonergic. We have examined this hypothesis directly using combined retrograde horseradish peroxidase/serotonin immunohistochemical staining. Cells of the ventral medulla contributing to the DLF were found to be largely non-serotonergic. This result was confirmed by two approaches: by the absence of a significant number of double-labeled cells following discrete horseradish peroxidase labeling of the DLF and by the persistence of double-labeled cells in animals with DLF lesions made rostral to intraspinal injections of horseradish peroxidase solutions. The conclusion is made that the previously described nucleus raphe magnus-DLF-dorsal horn pathway is predominantly non-serotonergic.
延髓腹侧的细胞，其轴突经背外侧索（DLF）下行至脊髓背角，在疼痛调节中起重要作用。先前的证据表明，该通路主要是血清素能的。我们使用逆行辣根过氧化物酶/血清素免疫组织化学联合染色直接检验了这一假设。发现对DLF有贡献的延髓腹侧细胞在很大程度上是非血清素能的。这一结果通过两种方法得到证实：对DLF进行离散辣根过氧化物酶标记后，未发现大量双标记细胞；在脊髓内注射辣根过氧化物酶溶液后，在延髓头端制造DLF损伤的动物中，双标记细胞持续存在。得出的结论是，先前描述的中缝大核-DLF-背角通路主要是非血清素能的。
