岛津CS 920密度计：用平行光束照射薄层色谱图及其对定量分析的影响。
The Shimadzu CS 920 densitometer: illumination of thin layer chromatograms with a parallel light beam and its effects on quantification.
作者信息
Huf F A
出版信息
Pharm Weekbl Sci. 1984 Feb 24;6(1):7-10. doi: 10.1007/BF01960190.
The Shimadzu CS 920 has shown to be an easy to handle flying-spot densitometer that enables rapid quantification of thin layer chromatograms. Linear calibration curves with a relative standard deviation of 3 to 4% in the slope can be obtained for Sudan Yellow on Merck H60 plates. However, contrary to the statements in the instrument manual, the 'linearizer' performance is not in agreement with the Kubelka and Munk theory. Consequently, the CS 920 can only be used after empirically determined adjustment of the 'linearizer'. Furthermore, errors in analysis can increase up to 30% by using TLC-plates with fluctuating layer thickness.
岛津CS 920已被证明是一种易于操作的飞点密度计，能够快速定量薄层色谱图。在默克H60板上对苏丹黄可获得斜率相对标准偏差为3%至4%的线性校准曲线。然而，与仪器手册中的说明相反，“线性化器”的性能与库贝尔卡和蒙克理论不一致。因此，CS 920只有在根据经验确定对“线性化器”进行调整后才能使用。此外，使用层厚波动的薄层色谱板时，分析误差可能会增加高达30%。
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