Epstein D J, Neeley W E
Clin Chem. 1984 Jun;30(6):847-50.
In discussing the principles of quantitative analysis in thin-layer media, we show that requirements for quantitative analysis are not satisfied when stained protein electrophoretic bands are scanned with a conventional rectangular-slit densitometer. We investigated a high-resolution densitometer based on a linear photodiode array as an alternative analytical tool, using stained electrophoretic bands of radio-labeled human serum albumin as a simplified model for results of serum protein electrophoresis. Identical protein bands scanned with both the high-resolution densitometer and a conventional densitometer were quantified with improved accuracy and precision by the new instrument. We also used the high-resolution densitometer to develop a computer model for performance characteristics of a rectangular-slit densitometer.
在讨论薄层介质定量分析的原理时，我们发现，当用传统的矩形狭缝密度计扫描染色的蛋白质电泳条带时，定量分析的要求无法得到满足。我们研究了一种基于线性光电二极管阵列的高分辨率密度计，将其作为一种替代分析工具，使用放射性标记的人血清白蛋白的染色电泳条带作为血清蛋白电泳结果的简化模型。用高分辨率密度计和传统密度计扫描的相同蛋白质条带，通过新仪器进行定量时，准确性和精密度都得到了提高。我们还使用高分辨率密度计开发了一个用于矩形狭缝密度计性能特征的计算机模型。
