诺氏疟原虫子孢子抗原：由感染性重组痘苗病毒表达

Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus.

作者信息

Smith G L, Godson G N, Nussenzweig V, Nussenzweig R S, Barnwell J, Moss B

出版信息

Science. 1984 Apr 27;224(4647):397-9. doi: 10.1126/science.6200932.

DOI:10.1126/science.6200932

PMID:6200932

Abstract

The gene coding for the circumsporozoite antigen of the malaria parasite Plasmodium knowlesi was inserted into the vaccinia virus genome under the control of a defined vaccinia virus promoter. Cells infected with the recombinant virus synthesized polypeptides of 53,000 to 56,000 daltons that reacted with monoclonal antibody against the repeating epitope of the malaria protein. Furthermore, rabbits vaccinated with the recombinant virus produced antibodies that bound specifically to sporozoites. These data provide evidence for expression of a cloned malaria gene in mammalian cells and illustrate the potential of vaccinia virus recombinants as live malaria vaccines.

摘要

将诺氏疟原虫疟原虫环子孢子抗原的编码基因，在特定痘苗病毒启动子的控制下插入痘苗病毒基因组。用重组病毒感染的细胞合成了分子量为53,000至56,000道尔顿的多肽，这些多肽与抗疟疾蛋白重复表位的单克隆抗体发生反应。此外，用重组病毒免疫的兔子产生了能与子孢子特异性结合的抗体。这些数据为克隆的疟疾基因在哺乳动物细胞中的表达提供了证据，并说明了痘苗病毒重组体作为活疟疾疫苗的潜力。