Langford C J, Edwards S J, Smith G L, Mitchell G F, Moss B, Kemp D J, Anders R F
Mol Cell Biol. 1986 Sep;6(9):3191-9. doi: 10.1128/mcb.6.9.3191-3199.1986.
We show that the subcellular location of foreign antigens expressed in recombinant vaccinia viruses influences their effectiveness as immunogens. Live recombinant viruses induced very poor antibody responses to a secreted repetitive plasmodial antigen (the S-antigen) in rabbits and mice. The poor response accords with epidemiological data suggesting that S-antigens are poorly immunogenic. Appending the transmembrane domain of a membrane immunoglobulin (immunoglobulin G1) to its carboxy terminus produced a hybrid S-antigen that was no longer secreted but was located on the surface of virus-infected cells. This recombinant virus elicited high antibody titers to the S-antigen. This approach will facilitate the use of live virus delivery systems to immunize against a wide range of foreign nonsurface antigens.
我们发现，重组痘苗病毒中表达的外源抗原的亚细胞定位会影响其作为免疫原的效力。活重组病毒在兔和小鼠体内诱导出对一种分泌型疟原虫重复抗原（S抗原）的非常微弱的抗体反应。这种微弱反应与流行病学数据相符，表明S抗原免疫原性较差。在其羧基末端附加膜免疫球蛋白（免疫球蛋白G1）的跨膜结构域，产生了一种不再分泌但位于病毒感染细胞表面的杂交S抗原。这种重组病毒引发了针对S抗原的高抗体滴度。这种方法将有助于利用活病毒递送系统针对多种外源非表面抗原进行免疫接种。
