Booth N A, Bennett B
Br J Haematol. 1984 Apr;56(4):545-56. doi: 10.1111/j.1365-2141.1984.tb02179.x.
A two-dimensional immunoelectrophoresis (2DIEP) method detects plasmin complexed to its major inhibitor, alpha 2-antiplasmin, in plasma in the blood of patients during (a) thrombolytic therapy with urokinase, (b) episodes of disseminated intravascular coagulation (DIC) with active fibrinolysis, and (c) episodes of fibrinolytic haemorrhage without evidence of DIC. Clearance of the complexes from the blood is rapid and their detection thus implies active plasmin generation at the time of blood sampling or within the preceding 24 h. Abolition of the complexes using tranexamic acid therapy allowed surgery without bleeding in two previously grossly haemorrhagic patients in group (c). Antithrombin III complexed with activated procoagulants was detected using a similar 2DIEP method in only two of four patients with DIC. Abnormalities of alpha 2-macroglobulin were detected on 2DIEP of plasma in the patients studied with proteolytic disorders; these did not appear to reflect complex formation.
二维免疫电泳（2DIEP）方法可检测患者血液血浆中与主要抑制剂α2 - 抗纤溶酶结合的纤溶酶，这些患者处于以下情况：（a）接受尿激酶溶栓治疗期间；（b）伴有活跃纤维蛋白溶解的弥散性血管内凝血（DIC）发作期间；（c）无DIC证据的纤维蛋白溶解出血发作期间。复合物从血液中的清除速度很快，因此其检测意味着在采血时或之前24小时内有活跃的纤溶酶生成。在组（c）中，两名先前严重出血的患者使用氨甲环酸治疗消除复合物后得以进行手术且未出血。在仅4名DIC患者中的2名中，使用类似的2DIEP方法检测到了与活化促凝剂结合的抗凝血酶III。在研究的患有蛋白水解紊乱的患者血浆二维免疫电泳中检测到α2 - 巨球蛋白异常；这些异常似乎并不反映复合物的形成。
