Wolosewick J J, De Mey J, Meininger V
Biol Cell. 1983;49(3):219-26. doi: 10.1111/j.1768-322x.1984.tb00240.x.
The polyethylene glycol (PEG) method for immunofluorescence localization of cytoskeletal antigens has been extended to the ultrastructural level using glutaraldehyde-fixed tissues and immunogold staining. Semithin sections of fixed tissue embedded in polyethylene glycol are divested of the PEG, exposed to purified antibodies (e.g., antiactin, antitubulin) and anti-IgG-colloidal gold. The sections may be processed by dehydration and critical-point drying, or reembedment in hydrophilic substances. Tubulin is demonstrated in the mitotic spindles of dividing spermatogonia, manchettes, axonemes and centrioles of developing spermatids, and in the Sertoli cell cytoplasm; actin localization is demonstrated in the myoid cells of the tunica propria, and smooth muscle cells of arterioles in the interstitial tissue. The results demonstrate the applicability and versatility of PEG embedding for immunocytochemistry.
用于细胞骨架抗原免疫荧光定位的聚乙二醇（PEG）方法已通过使用戊二醛固定组织和免疫金染色扩展到超微结构水平。包埋在聚乙二醇中的固定组织半薄切片去除PEG，暴露于纯化抗体（如抗肌动蛋白、抗微管蛋白）和抗IgG胶体金。切片可通过脱水和临界点干燥处理，或重新包埋在亲水性物质中。在分裂的精原细胞的有丝分裂纺锤体、发育中的精子细胞的袖套、轴丝和中心粒以及支持细胞胞质中显示出微管蛋白；在固有层的肌样细胞和间质组织中小动脉的平滑肌细胞中显示出肌动蛋白定位。结果证明了PEG包埋在免疫细胞化学中的适用性和多功能性。
