• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Immuno gold staining (IGS) for electron microscopical demonstration of glial fibrillary acidic (GFA) protein in LR white embedded tissue.

作者信息

Gräber M B, Kreutzberg G W

出版信息

Histochemistry. 1985;83(6):497-500. doi: 10.1007/BF00492450.

DOI:10.1007/BF00492450
PMID:3910622
Abstract

Post-embedding immunocytochemical staining methods using gold have so far failed to label intermediate filament antigens in situ in epon or araldite embedded tissue. We have now applied the post-embedding immuno gold staining (IGS) technique for LR White embedded tissue. Glial fibrillary acidic (GFA) protein immunoreactivity was clearly demonstrated electron microscopically on astrocytic filaments of rat cerebellum in situ.

摘要

相似文献

1
Immuno gold staining (IGS) for electron microscopical demonstration of glial fibrillary acidic (GFA) protein in LR white embedded tissue.
Histochemistry. 1985;83(6):497-500. doi: 10.1007/BF00492450.
2
Effects of prolonged ethanol exposure on the glial fibrillary acidic protein-containing intermediate filaments of astrocytes in primary culture: a quantitative immunofluorescence and immunogold electron microscopic study.长期乙醇暴露对原代培养星形胶质细胞中含胶质纤维酸性蛋白的中间丝的影响：定量免疫荧光和免疫金电子显微镜研究
J Histochem Cytochem. 1989 Feb;37(2):229-40. doi: 10.1177/37.2.2642942.
3
An epithelium-type cytoskeleton in a glial cell: astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes.神经胶质细胞中的上皮型细胞骨架：两栖类视神经的星形胶质细胞含有细胞角蛋白丝，并通过桥粒相连。
J Cell Biol. 1989 Aug;109(2):705-16. doi: 10.1083/jcb.109.2.705.
4
On-grid immunogold labeling of glial intermediate filaments in epoxy-embedded tissue.环氧树脂包埋组织中神经胶质中间丝的网格上免疫金标记
Am J Anat. 1989 Jun-Jul;185(2-3):335-41. doi: 10.1002/aja.1001850228.
5
Vimentin and glial fibrillary acidic protein are codistributed in the same intermediate filament system of malignant glioma cells in vivo. A double-labeling immunoelectron-microscopical study.波形蛋白和胶质纤维酸性蛋白在体内恶性胶质瘤细胞的同一中间丝系统中共分布。一项双标记免疫电子显微镜研究。
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(1):67-70. doi: 10.1007/BF02890003.
6
Immunogold electron microscopic localization of glial fibrillary acidic protein (GFAP) in neurohypophyseal pituicytes and tanycytes of the Mongolian gerbil (Meriones unguiculatus).
Histochemistry. 1989;91(4):333-7. doi: 10.1007/BF00493009.
7
Immunocytochemical localization of cysteine sulfinate decarboxylase in astrocytes in the cerebellum and hippocampus: a quantitative double immunofluorescence study with glial fibrillary acidic protein and S-100 protein.小脑和海马体中星形胶质细胞内半胱氨酸亚磺酸盐脱羧酶的免疫细胞化学定位：一项使用胶质纤维酸性蛋白和S-100蛋白的定量双重免疫荧光研究
Neuroscience. 1996 Nov;75(2):619-33. doi: 10.1016/0306-4522(96)00256-4.
8
Ultrastructure of intermediate filaments of nestin- and vimentin-immunoreactive astrocytes in organotypic slice cultures of hippocampus.海马器官型切片培养中巢蛋白和波形蛋白免疫反应性星形胶质细胞中间丝的超微结构
J Struct Biol. 1997 Oct;120(1):61-8. doi: 10.1006/jsbi.1997.3900.
9
An ultrastructural and immunocytochemical study of astrocytic differentiation in vitro: changes in the composition and distribution of the cellular cytoskeleton.体外星形胶质细胞分化的超微结构和免疫细胞化学研究：细胞骨架组成和分布的变化
J Neuroimmunol. 1982 Jun;2(3-4):235-60. doi: 10.1016/0165-5728(82)90058-3.
10
Immunoelectron microscopic localization of keratin filaments: a post-embedding technique.
J Cutan Pathol. 1988 Aug;15(4):223-5. doi: 10.1111/j.1600-0560.1988.tb00548.x.

引用本文的文献

1
Cuprizone Administration Alters the Iron Metabolism in the Mouse Model of Multiple Sclerosis.铜替佐胺处理改变多发性硬化症小鼠模型的铁代谢。
Cell Mol Neurobiol. 2018 Jul;38(5):1081-1097. doi: 10.1007/s10571-018-0578-5. Epub 2018 Feb 20.
2
A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays.免疫荧光和三重免疫金测定中抗体信号增强的简单解决方案。
Histochem Cell Biol. 2016 Oct;146(4):421-30. doi: 10.1007/s00418-016-1447-2. Epub 2016 May 17.
3
Ultrastructural characteristics of glial fibrillary acidic protein expression in epoxy resin-embedded human brain tumors.

本文引用的文献

1
FIXATION OF THE CENTRAL NERVOUS SYSTEM FROM ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION. I. PRESERVATION WITH ALDEHYDE PERFUSATES VERSUS DIRECT PERFUSION WITH OSMIUM TETROXIDE WITH SPECIAL REFERENCE TO MEMBRANES AND THE EXTRACELLULAR SPACE.通过醛灌注进行电子显微镜检查时中枢神经系统的固定。I. 醛灌注液保存与四氧化锇直接灌注的比较，特别涉及膜和细胞外空间
J Ultrastruct Res. 1965 Feb;12:160-86. doi: 10.1016/s0022-5320(65)80014-4.
2
High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method.采用免疫金染色（IGS）方法对微管蛋白进行高分辨率光镜和电镜定位。
Cell Biol Int Rep. 1981 Sep;5(9):889-99. doi: 10.1016/0309-1651(81)90204-6.
3
环氧树脂包埋的人脑肿瘤中胶质纤维酸性蛋白表达的超微结构特征
Acta Neuropathol. 1993;86(3):295-301. doi: 10.1007/BF00304145.
4
Golgi-like immunostaining of pituicytes and tanycytes positive for glial fibrillary acidic protein in the neurohypophysis of the Mongolian gerbil (Meriones unguiculatus).在蒙古沙鼠（长爪沙鼠）神经垂体中，对胶质纤维酸性蛋白呈阳性反应的垂体细胞和伸展细胞的高尔基体样免疫染色。
Histochemistry. 1987;87(6):585-95. doi: 10.1007/BF00492475.
5
Use and abuse of LR White.LR White的使用与滥用。
Histochem J. 1987 Feb;19(2):118-20. doi: 10.1007/BF01682756.
6
Immunogold electron microscopic localization of glial fibrillary acidic protein (GFAP) in neurohypophyseal pituicytes and tanycytes of the Mongolian gerbil (Meriones unguiculatus).
Histochemistry. 1989;91(4):333-7. doi: 10.1007/BF00493009.
7
Ultrastructural immunocytochemistry of glia cells. Double labeling studies using LR White embedding and colloidal gold.神经胶质细胞的超微结构免疫细胞化学。使用LR White包埋和胶体金的双重标记研究。
Histochemistry. 1987;87(3):201-7. doi: 10.1007/BF00492410.
8
Astrocyte gene expression in Creutzfeldt-Jakob disease.克雅氏病中的星形胶质细胞基因表达。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5937-41. doi: 10.1073/pnas.84.16.5937.
9
LR Gold embedding of nervous tissue for immunoelectron microscopy studies.用于免疫电子显微镜研究的神经组织LR金包埋法。
Histochemistry. 1992;97(5):413-9. doi: 10.1007/BF00270388.
A review of the colloidal gold marker system.
胶体金标记系统综述。
Scan Electron Microsc. 1980(Pt 2):133-46.
4
Laminin in cultured mouse C1300 neuroblastoma cells: immunocytochemical localization by pre- and postembedding electron microscope procedures.培养的小鼠C1300神经母细胞瘤细胞中的层粘连蛋白：通过包埋前和包埋后电子显微镜程序进行免疫细胞化学定位
J Histochem Cytochem. 1983 Jun;31(6):755-64. doi: 10.1177/31.6.6841971.
5
Attachment of vimentin filaments to desmosomal plaques in human meningiomal cells and arachnoidal tissue.波形蛋白丝与人类脑膜瘤细胞和蛛网膜组织中桥粒斑的附着。
J Cell Biol. 1984 Mar;98(3):1072-81. doi: 10.1083/jcb.98.3.1072.
6
An intermediate filament-associated protein, p50, recognized by monoclonal antibodies.一种中间丝相关蛋白，p50，可被单克隆抗体识别。
J Cell Biol. 1983 Nov;97(5 Pt 1):1507-14. doi: 10.1083/jcb.97.5.1507.
7
Immunocytochemical localization of tubulin with colloidal gold in cilia of rabbit tracheal epithelial cultures.兔气管上皮培养物纤毛中微管蛋白与胶体金的免疫细胞化学定位
Tissue Cell. 1983;15(1):39-45. doi: 10.1016/0040-8166(83)90032-0.
8
Ultrastructural localization of tubulin and actin in polyethylene glycol-embedded rat seminiferous epithelium by immunogold staining.通过免疫金染色对聚乙二醇包埋的大鼠生精上皮中微管蛋白和肌动蛋白进行超微结构定位。
Biol Cell. 1983;49(3):219-26. doi: 10.1111/j.1768-322x.1984.tb00240.x.
9
Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides.对胶质纤维酸性（GFA）蛋白以及神经丝三联体多肽中的每一种具有特异性的单克隆抗体。
Differentiation. 1983;25(2):193-203. doi: 10.1111/j.1432-0436.1984.tb01355.x.
10
Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.脱水和包埋温度会影响微管蛋白的抗原特异性以及通过蛋白A-胶体金技术进行的免疫标记。
J Histochem Cytochem. 1983 Dec;31(12):1380-4. doi: 10.1177/31.12.6195215.