Wolosewick J J
Cell Tissue Res. 1984;236(3):517-25. doi: 10.1007/BF00217218.
Blood cells proliferate extravascularly in the bone marrow and enter the circulation by migrating through endothelial cells of venous blood sinuses. This migration, or diapedesis, was suspected to involve actin. To test for the presence and distribution of actin, sections of rat bone marrow were examined by indirect immunocyto-chemistry. Affinity purified rabbit antichicken gizzard actin antibody, and goat-antirabbit IgG-FITC, or goat antirabbit IgG colloidal gold probes were used. The migrating cell contacts the endothelial cell and forms a podosome (a cortical bleb). Immunocytochemistry shows this region to contain actin. As diapedesis proceeds the podosome deforms, then breaches the endothelial cell. At this time the anterior portion of the leukocyte shows heavy labeling for actin. When the migratory cell traverses approximately half of its length through the endothelial cell, actin appears prominent in the caudal region of the cell. The immunocytochemical data suggest that actin is nonrandomly distributed in leukocytes undergoing diapedesis and may be a component of the force-generating mechanism responsible for this transcellular migratory event.
血细胞在骨髓血管外增殖，并通过迁移穿过静脉血窦的内皮细胞进入循环系统。这种迁移，即血细胞渗出，被怀疑与肌动蛋白有关。为了检测肌动蛋白的存在和分布，通过间接免疫细胞化学方法检查了大鼠骨髓切片。使用了亲和纯化的兔抗鸡砂囊肌动蛋白抗体，以及山羊抗兔IgG-FITC或山羊抗兔IgG胶体金探针。迁移细胞与内皮细胞接触并形成一个足体（一种皮质小泡）。免疫细胞化学显示该区域含有肌动蛋白。随着血细胞渗出的进行，足体变形，然后穿透内皮细胞。此时白细胞的前部显示出强烈的肌动蛋白标记。当迁移细胞穿过内皮细胞约一半长度时，肌动蛋白在细胞的尾部区域显得突出。免疫细胞化学数据表明，肌动蛋白在进行血细胞渗出的白细胞中呈非随机分布，并且可能是负责这种跨细胞迁移事件的力产生机制的一个组成部分。
