Lema M, Otero J, Marcó J
Department of Physiology, Faculty of Biology, University of Santiago, Galicia, Spain.
J Chromatogr. 1991 Jun 28;547(1-2):113-20. doi: 10.1016/s0021-9673(01)88634-9.
A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design.
本文描述了一种采用电化学检测的新型高效液相色谱法，该方法可同时测定来自单个大鼠脑内显微切割核中的多巴胺、血清素、3,4-二羟基苯乙酸、3-甲氧基-4-羟基苯乙酸、5-羟色氨酸和5-羟基吲哚乙酸。无需样品预处理步骤。通过一个简单的有限优化程序（涉及双参数析因设计）对分离度和分析时间进行了优化。
