Raska I, Valouch P, Armbruster B L, Hinterberger M, Malý A, Vorlícek J, Smetana K, Kellenberger E
Histochem J. 1985 Aug;17(8):925-38. doi: 10.1007/BF01004187.
The postembedding localization of rRNA was investigated in ultrathin sections of HeLa cells, rat liver and Xenopus laevis oocytes by means of the monoclonal antibody to rRNA and protein A-gold technique. The incidence of gold particles was highest in nucleoli and cytoplasmic areas containing ribosomes. The chromosomes were labelled less than the surrounding cytoplasm in mitotic HeLa cells. In nucleoli of HeLa cells and rat hepatocytes, the labelling of areas containing ribonucleoprotein components was greater than the labelling of fibrillar centres. In segregated nucleoli of X. laevis oocytes, the labelling of the granular region substantially exceeded that of the fibrillar regions. The incidence of nucleoplasmic gold particles in interphasic HeLa cells was found to be slightly increased in the vicinity of nucleoli. The labelling of clusters of interchromatin granules in rat hepatocytes was not significantly different from that of the rest of the nucleophasmic interchromatin spaces.
利用针对rRNA的单克隆抗体和蛋白A-金技术，对HeLa细胞、大鼠肝脏和非洲爪蟾卵母细胞的超薄切片进行了rRNA的包埋后定位研究。金颗粒的发生率在核仁和含有核糖体的细胞质区域最高。在有丝分裂的HeLa细胞中，染色体的标记少于周围的细胞质。在HeLa细胞和大鼠肝细胞的核仁中，含有核糖核蛋白成分区域的标记大于纤维中心的标记。在非洲爪蟾卵母细胞分离的核仁中，颗粒区域的标记明显超过纤维区域的标记。发现在间期HeLa细胞核质中金颗粒的发生率在核仁附近略有增加。大鼠肝细胞中染色质间颗粒簇的标记与其余核质染色质间空间的标记无显著差异。
