Suppr超能文献

对H-2k和H-2b脾细胞进行三硝基苯基修饰可增强对Kk样决定簇的血清学检测。

Trinitrophenyl modification of H-2k and H-2b spleen cells results in enhanced serological detection of Kk-like determinants.

作者信息

Levy R B, Dower S K, Shearer G M, Segal D M

出版信息

J Exp Med. 1984 May 1;159(5):1464-72. doi: 10.1084/jem.159.5.1464.

DOI:10.1084/jem.159.5.1464
PMID:6201586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2187294/
Abstract

Several anti-H-2Kk but not anti-H-2Dd monoclonal antibodies (mAb) exhibited enhanced binding to B10.A murine spleen cells after modification of the cells with trinitrobenzene sulfonate (TNBS). The number of antibody molecules bound to TNP-modified B10.A spleen cells increased by a factor of two or more. The same anti-2Kk mAb that exhibited enhanced binding to modified B10.A cells did not bind to unmodified C57BL/10 spleen cells, as expected, but did bind to TNP-modified C57BL/10 spleen cells. This TNP-dependent binding was not a result of cross-reactions with cell surface TNP groups nor with Fc receptors. TNP modification of a variant cell line that does not express class I H-2 products did not result in enhanced binding by these mAb. These findings can account for preferential recognition of TNP-Kk by B10.A and B10.BR CTL, and also for cross-reactive lysis by C57BL/10 CTL stimulated by C57BL/10-TNP against unmodified H-2Kk targets.

摘要

在用三硝基苯磺酸（TNBS）修饰细胞后，几种抗H-2Kk单克隆抗体（mAb）而非抗H-2Dd单克隆抗体与B10.A小鼠脾细胞的结合增强。与经三硝基苯（TNP）修饰的B10.A脾细胞结合的抗体分子数量增加了两倍或更多。正如预期的那样，与修饰后的B10.A细胞结合增强的相同抗2Kk单克隆抗体不与未修饰的C57BL/10脾细胞结合，但确实与经TNP修饰的C57BL/10脾细胞结合。这种TNP依赖性结合不是与细胞表面TNP基团或Fc受体交叉反应的结果。对不表达I类H-2产物的变异细胞系进行TNP修饰，不会导致这些单克隆抗体的结合增强。这些发现可以解释B10.A和B10.BR细胞毒性T淋巴细胞（CTL）对TNP-Kk的优先识别，以及C57BL/10-TNP刺激的C57BL/10 CTL对未修饰的H-2Kk靶标的交叉反应性裂解。

相似文献

1
Trinitrophenyl modification of H-2k and H-2b spleen cells results in enhanced serological detection of Kk-like determinants.对H-2k和H-2b脾细胞进行三硝基苯基修饰可增强对Kk样决定簇的血清学检测。
J Exp Med. 1984 May 1;159(5):1464-72. doi: 10.1084/jem.159.5.1464.
2
Detection of shared H-2Kk and H-2Dk antigens that function as self determinants for cell-mediated lympholysis to trinitrophenyl-self.检测作为针对三硝基苯基自身的细胞介导性淋巴细胞溶解的自身决定簇起作用的共享H-2Kk和H-2Dk抗原。
J Immunol. 1981 Apr;126(4):1522-5.
3
H-2-linked genetic control of murine T-cell-mediated lympholysis to autologous cells modified with low concentrations of trinitrobenzene sulfonate.H-2相关的小鼠T细胞介导的对用低浓度三硝基苯磺酸修饰的自体细胞的淋巴细胞溶解的遗传控制。
J Exp Med. 1979 Jun 1;149(6):1407-23. doi: 10.1084/jem.149.6.1407.
4
Defining cytolytic T lymphocyte recognition of chemically modified self. I. Response to trinitrophenyl-H-2Kk.定义化学修饰自身的细胞溶解性T淋巴细胞识别。I. 对三硝基苯基-H-2Kk的反应。
J Immunol. 1985 Sep;135(3):1564-72.
5
Production and characterization of T-cell clones specific for trinitrophenyl (TNP)-modified syngeneic spleen cells (TNP-self).针对三硝基苯基（TNP）修饰的同基因脾细胞（TNP-自身）的T细胞克隆的产生与特性分析
Indian J Exp Biol. 1989 Oct;27(10):843-8.
6
Hapten-specific cytotoxic T cell clones undergo somatic variation of their antigen recognition specificity.半抗原特异性细胞毒性T细胞克隆会经历其抗原识别特异性的体细胞变异。
Eur J Immunol. 1986 Jun;16(6):631-9. doi: 10.1002/eji.1830160608.
7
H2Kk can influence whether cytotoxic T lymphocytes recognize trinitrophenyl in association with H2Dk unique or H-2Kk and H-2Dk shared self determinants.H2Kk能够影响细胞毒性T淋巴细胞是否识别与H2Dk独特的或H-2Kk和H-2Dk共有的自身决定簇相关联的三硝基苯。
J Immunol. 1982 Sep;129(3):1189-93.
8
Regulation of the hapten-specific T cell response. I. Preferential induction of hyporesponsiveness to the D-end of the major histocompatibility complex in the hapten-specific cytotoxic T cell response.半抗原特异性T细胞应答的调节。I. 在半抗原特异性细胞毒性T细胞应答中对主要组织相容性复合体D端低反应性的优先诱导。
J Immunol. 1983 Nov;131(5):2184-9.
9
Cytotoxic T lymphocyte responses in allogeneic radiation bone marrow chimeras. The chimeric host strictly dictates the self-repertoire of Ia-restricted T cells but not H-2K/D-restricted T cells.同种异体辐射骨髓嵌合体中的细胞毒性T淋巴细胞反应。嵌合宿主严格决定Ia限制性T细胞的自身 repertoire，但不决定H-2K/D限制性T细胞的自身 repertoire。
J Exp Med. 1982 Dec 1;156(6):1650-64. doi: 10.1084/jem.156.6.1650.
10
Regulation of T-cell-mediated lympholysis by the murine major histocompatibility complex. I. Preferential in vitro responses to trinitrophenyl-modified self K- and D-coded gene products in parental and F1 hybrid mouse strains.小鼠主要组织相容性复合体对T细胞介导的淋巴细胞溶解的调节。I. 亲代和F1杂交小鼠品系对三硝基苯基修饰的自身K和D编码基因产物的体外优先反应。
J Exp Med. 1979 Jun 1;149(6):1379-92. doi: 10.1084/jem.149.6.1379.

引用本文的文献

1
Production of target-specific effector cells using hetero-cross-linked aggregates containing anti-target cell and anti-Fc gamma receptor antibodies.使用含有抗靶细胞抗体和抗Fcγ受体抗体的异源交联聚集体产生靶向特异性效应细胞。
J Exp Med. 1984 Dec 1;160(6):1686-701. doi: 10.1084/jem.160.6.1686.
2
Anti-MHC immunity detected prior to intentional alloimmunization. III. Natural autoreactive H-2-specific antibodies.在有意进行同种免疫之前检测到的抗MHC免疫。III. 天然自身反应性H-2特异性抗体。
Immunogenetics. 1985;21(5):491-504. doi: 10.1007/BF00430932.
3
H-2-specific antibodies induced by injection of syngeneic leukemia cells.通过注射同基因白血病细胞诱导产生的H-2特异性抗体。
Immunogenetics. 1987;25(4):215-21. doi: 10.1007/BF00404690.
4
Polymorphic and autoreactive H-2-specific monoclonal antibody isolated after injections of syngeneic Sendai virus-coated lymphocytes.注射同基因仙台病毒包被的淋巴细胞后分离得到的多态性和自身反应性H-2特异性单克隆抗体。
Immunogenetics. 1986;24(6):402-8. doi: 10.1007/BF00377959.
5
Allorestricted cytotoxic T cells. Large numbers of allo-H-2Kb-restricted antihapten and antiviral cytotoxic T cell populations clonally develop in vitro from murine splenic precursor T cells.所有受限制的细胞毒性T细胞。大量受H-2Kb限制的抗半抗原和抗病毒细胞毒性T细胞群体在体外从鼠脾前体T细胞中克隆发育而来。
J Exp Med. 1985 Aug 1;162(2):592-606. doi: 10.1084/jem.162.2.592.
6
Immunization with syngeneic Sendai virus-infected cells induce no MHC-restricted antibodies but antibodies specific for H-2 class I determinants.用同基因仙台病毒感染的细胞进行免疫接种不会诱导产生MHC限制性抗体，但会诱导产生针对H-2 I类决定簇的特异性抗体。
Immunogenetics. 1989;29(2):108-11. doi: 10.1007/BF00395858.

本文引用的文献

1
H-2-linked genetic control of murine cell-mediated lympholysis to autologous cells modified with high and low concentrations of fluorescein isothiocyanate.H-2连锁的对用高浓度和低浓度异硫氰酸荧光素修饰的自体细胞的小鼠细胞介导的淋巴细胞溶解的遗传控制。
Cell Immunol. 1981 Mar 15;59(1):97-105. doi: 10.1016/0008-8749(81)90437-8.
2
Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex.主要结构：小鼠H-2主要组织相容性复合体移植抗原的分析
Annu Rev Biochem. 1981;50:1025-52. doi: 10.1146/annurev.bi.50.070181.005113.
3
Low responsiveness to Dk or Db plus vaccinia virus or to Kk plus lymphocytic choriomeningitis virus assessed by availability of D or K products.根据D或K产物的可获得性评估，对Dk或Db加痘苗病毒或对Kk加淋巴细胞性脉络丛脑膜炎病毒的低反应性。
Tissue Antigens. 1981 May;17(5):507-17. doi: 10.1111/j.1399-0039.1981.tb00738.x.
4
Cell-mediated lympholysis responses against autologous cells modified with haptenic sulfhydryl reagents. II. Analysis of the genetic control and H-2-restricted pattern of cytotoxic responses to sulfhydryl and amino reactive reagents.针对经半抗原巯基试剂修饰的自体细胞的细胞介导的淋巴细胞溶解反应。II. 对巯基和氨基反应性试剂细胞毒性反应的遗传控制及H-2限制模式的分析
J Immunol. 1981 Aug;127(2):529-34.
5
T cells respond preferentially to antigens that are similar to self H-2.T细胞优先对与自身H-2相似的抗原作出反应。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1843-7. doi: 10.1073/pnas.78.3.1843.
6
Cytotoxic T lymphocyte responses by chimeric thymocytes. Self-recognition is determined early in T cell development.嵌合胸腺细胞引发的细胞毒性T淋巴细胞反应。自我识别在T细胞发育早期就已确定。
J Exp Med. 1981 Jan 1;153(1):13-29. doi: 10.1084/jem.153.1.13.
7
Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.抗小鼠主要组织相容性复合体（MHC）抗原的单克隆抗体。III. 与H-2b单倍型抗原发生反应的杂交瘤抗体揭示了同种型表达的遗传控制。
J Immunol. 1981 Jan;126(1):317-21.
8
Selective turnover and shedding of H-2K and H-2D antigens is controlled by the major histocompatibility complex. Implications for H-2-restricted recognition.H-2K和H-2D抗原的选择性周转和脱落受主要组织相容性复合体控制。对H-2限制性识别的影响。
J Exp Med. 1980 Oct 1;152(4):783-95. doi: 10.1084/jem.152.4.783.
9
Preferential response patterns of cytotoxic T lymphocytes specific for fluorescein isothiocyanate-[FITC] modified autologous cells.针对异硫氰酸荧光素-[FITC]修饰的自体细胞的细胞毒性T淋巴细胞的优先反应模式。
J Immunol. 1982 Feb;128(2):551-5.
10
Cross-reactive recognition of alloantigens and certain B6 mutant antigens by antigen-specific and polyclonally activated cytotoxic effector cells.抗原特异性及多克隆激活的细胞毒性效应细胞对同种异体抗原和某些B6突变抗原的交叉反应性识别。
Cell Immunol. 1983 Aug;80(1):163-71. doi: 10.1016/0008-8749(83)90103-x.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验