Zinkernagel R M, Callahan G N
Tissue Antigens. 1981 May;17(5):507-17. doi: 10.1111/j.1399-0039.1981.tb00738.x.
The possibility was examined that K or D regulated responsiveness of virus specific cytotoxic T cells was due to the virus-specific and differential effects on expression and/or accessibility of H-2K or D products on virus-infected target cells. Cells from established lines of fibroblasts kept in tissue culture, uninfected, infected with either vaccinia virus, with lymphocytic choriomeningitis virus (LCMV) or with both LCMV plus vaccinia virus, were compared with respect to expression of Kk, Dk and Db. H-2K or D expression was assessed by: 1) absorption of defined anti-K or anti-D antisera: 2) susceptibility to alloreactive cytotoxic T cells; and 3) susceptibility to K or D restricted virus-specific cytotoxic T cells. In all 3 tests, no virus-specific effect on Kk, Dk or Db expression on all target cells (uninfected, LCMV, or vaccinia virus infected, or doubly infected) was noticed. Most importantly, H-2k target cells infected with both LCMV plus vaccinia virus, that were not lysed by low-responder Dk-restricted vaccinia specific T cells were lysed by high-responder Dk restricted LCMV-immune T cells; in contrast, these targets were lysed by Kk-restricted vaccinia specific T cells but only poorly by Kk-restricted LCMV-immune T cells. Thus, expression or accessibility of Dk could not have limited responsiveness to vaccinia virus on this target cell since Dk was accessible to Dk-restricted LCMV-specific T cells; a similar argument can be made for the accessibility of Kk. These experiments suggest that expression and/or accessibility of Kk, Dk or Db on target cells does not explain the virus- and K or D dependent responsiveness differences of virus-specific effector T cells.
研究了K或D调节病毒特异性细胞毒性T细胞反应性的可能性，即其是否归因于对病毒感染靶细胞上H-2K或D产物的表达和/或可及性产生的病毒特异性和差异性影响。将组织培养中保存的成纤维细胞系的细胞进行比较，这些细胞未感染、感染痘苗病毒、感染淋巴细胞性脉络丛脑膜炎病毒（LCMV）或同时感染LCMV和痘苗病毒，比较它们Kk、Dk和Db的表达情况。通过以下方式评估H-2K或D的表达：1）用特定的抗K或抗D抗血清进行吸收；2）对同种反应性细胞毒性T细胞的敏感性；3）对K或D限制性病毒特异性细胞毒性T细胞的敏感性。在所有这三项测试中，未发现对所有靶细胞（未感染、LCMV感染、痘苗病毒感染或双重感染）上的Kk、Dk或Db表达有病毒特异性影响。最重要的是，同时感染LCMV和痘苗病毒的H-2k靶细胞，未被低反应性Dk限制性痘苗特异性T细胞裂解，但被高反应性Dk限制性LCMV免疫T细胞裂解；相反，这些靶细胞被Kk限制性痘苗特异性T细胞裂解，但被Kk限制性LCMV免疫T细胞裂解的程度较差。因此，Dk的表达或可及性不可能限制该靶细胞对痘苗病毒的反应性，因为Dk可被Dk限制性LCMV特异性T细胞识别；对于Kk的可及性也可作类似推断。这些实验表明，靶细胞上Kk、Dk或Db的表达和/或可及性并不能解释病毒特异性效应T细胞的病毒依赖性和K或D依赖性反应性差异。
