Van Muijen G N, Ruiter D J, Warnaar S O
Department of Pathology, University Medical Center, Leiden, The Netherlands.
Lab Invest. 1987 Oct;57(4):359-69.
Tissues from human fetuses (12 to 14 weeks) were studied by using immunohistochemical methods, with special emphasis on coexpression of intermediate filaments. Well-characterized antibodies, monoclonal as well as polyclonal were used. Indirect immunoperoxidase staining disclosed simultaneous expression of cytokeratin and vimentin, or vimentin and desmin in several tissues, whereas some other tissues coexpressed three classes of intermediate filaments, i.e., cytokeratin, vimentin, and desmin. Coexpression of cytokeratin and vimentin was seen in immature tubules of the kidney localized in the blastema and in one case in a small area of the epithelium of the tongue. Coexpression of vimentin and desmin was found in stromal cells of the medulla of the kidney, in stromal cells of the decidua (maternal tissue) and in muscle cells in blood vessels of small intestine, kidney and decidua. Coexpression of cytokeratin, vimentin, and desmin was present in mesothelial cells of serosa, pleura and pericardium, in stroma of umbilical cord and placental villi, in muscle cells of small intestine, tongue, and heart, and in muscle cells of blood vessels of lung, heart, umbilical cord, and placental villi. Mesothelium and reactive submesothelial stroma cells also coexpressed three classes of intermediate filament polypeptides. In some cases, immunoperoxidase results were confirmed by double labeling immunofluorescence microscopy or by immunoblotting experiments. The results of this study indicate that coexpression of different types of intermediate filaments is a more general phenomenon in fetal tissues than previously realized and it also occurs in some reactive proliferative lesions in the adult.
采用免疫组织化学方法对人胎儿（12至14周）组织进行研究，特别关注中间丝的共表达情况。使用了特性明确的抗体，包括单克隆抗体和多克隆抗体。间接免疫过氧化物酶染色显示，在多种组织中细胞角蛋白和波形蛋白、波形蛋白和结蛋白同时表达，而其他一些组织则共表达三类中间丝，即细胞角蛋白、波形蛋白和结蛋白。在位于胚基的肾未成熟小管以及一例舌上皮小区域中可见细胞角蛋白和波形蛋白的共表达。波形蛋白和结蛋白的共表达见于肾髓质的基质细胞、蜕膜（母体组织）的基质细胞以及小肠、肾和蜕膜血管的肌细胞中。细胞角蛋白、波形蛋白和结蛋白的共表达存在于浆膜、胸膜和心包膜的间皮细胞、脐带和胎盘绒毛的基质、小肠、舌和心脏的肌细胞以及肺、心脏、脐带和胎盘绒毛血管的肌细胞中。间皮和反应性间皮下基质细胞也共表达三类中间丝多肽。在某些情况下，免疫过氧化物酶结果通过双标记免疫荧光显微镜或免疫印迹实验得到证实。本研究结果表明，不同类型中间丝的共表达在胎儿组织中是一种比以往认识更为普遍的现象，并且在成人的一些反应性增殖性病变中也会出现。
