Stähli C, Takács B, Kocyba C
Mol Immunol. 1983 Oct;20(10):1095-7. doi: 10.1016/0161-5890(83)90118-9.
Sixteen monoclonal antibodies specific for thymosin alpha 1 [Low et al., J. biol. Chem. 254, 981-986 (1979)] obtained from two fusions with the spleens of three mice [Stähli et al., Meth. Enzym. 92, 26-36 (1982b)] all react with an epitope in the C-terminal half of thymosin alpha 1. Human and foetal calf serum contain substances which cross-react with this epitope. A simple procedure to selectively remove the cross-reactive material and a sensitive RIA are described.
从三只小鼠脾脏进行两次融合实验[施塔赫利等人，《酶学方法》92，26 - 36(1982b)]获得的16种针对胸腺素α1的单克隆抗体[洛等人，《生物化学杂志》254，981 - 986(1979)]均与胸腺素α1 C端的一个表位发生反应。人血清和胎牛血清中含有与该表位发生交叉反应的物质。本文描述了一种选择性去除交叉反应物质的简单方法以及一种灵敏的放射免疫分析法。
