Influence of medium composition on the production of cephamycins by S. griseus and S. lactamdurans and the characterization of their growth.

The process of cephamycin biosynthesis by S. griseus and S. lactamdurans strains was investigated. The antibiotic activities of both strains in thirteen media elaborated in our laboratory were compared with those in nine media described in the literature. The best medium was that one which contained 20 g/dcm3 of glucose and 30 g/dcm3 of corn steep liquor. In this medium after a two stage shaken culture fermentation both strains showed specific growth rate from 0.027 to 0.034 h-1 and biomass yield about 5 g of dry weight per 1 dcm3. Under these conditions the highest activity was achieved after 96 hours of the fermentation.