Malina H Z, Grudziński S K, Zalega U D
Acta Microbiol Pol. 1984;33(3-4):227-37.
Streptomyces griseus NRRL 3851 and Streptomyces lactamdurans NRRL 3802 are well known as producers of cephamycins A, B and C, respectively. Antibiotics produced by their highly active mutants: S. griseus G-12 (termed X-12-1 and X-12-2) and S. lactamdurans L-2 (termed X-2) have been isolated and found to belong also to the cephamycin family. Their IR spectra revealed beta-lactam absorption at 1750 cm-1 and their 1H NMR spectra demonstrated the presence of protons attached to 7-methoxycephem skeleton and, in addition, signals attributable to one vinyl proton and four aromatic protons. The chemical shift of the aromatic protons indicated meta substitution in the benzene ring of the antibiotic X-12-1, and para substitution in the benzene ring of the antibiotics X-12-2 and X-2. The product of acid hydrolysis of X-2 was shown to be identical with p-hydroxy-alpha-methoxycinnamic acid.
灰色链霉菌NRRL 3851和内酰胺链霉菌NRRL 3802分别是头孢霉素A、B和C的著名产生菌。由它们的高活性突变体产生的抗生素：灰色链霉菌G-12（称为X-12-1和X-12-2）和内酰胺链霉菌L-2（称为X-2）已被分离出来，发现也属于头孢霉素家族。它们的红外光谱显示在1750 cm-1处有β-内酰胺吸收，它们的1H NMR光谱表明存在与7-甲氧基头孢烯骨架相连的质子，此外，还有一个乙烯基质子和四个芳族质子的信号。芳族质子的化学位移表明抗生素X-12-1的苯环为间位取代，抗生素X-12-2和X-2的苯环为对位取代。X-2的酸水解产物被证明与对羟基-α-甲氧基肉桂酸相同。
