Weisgerber C, Jann B, Jann K
Eur J Biochem. 1984 May 2;140(3):553-6. doi: 10.1111/j.1432-1033.1984.tb08137.x.
The chemical structure of the outer (hexose) regions of the core oligosaccharide from Escherichia coli 09 with the complete R1 core, and from a R1-derived rfe mutant were analyzed using compositional analysis, methylation and gas chromatography/mass spectrometry. It was found that, in contrast to the branched outer region of the R1 core, the outer region of the core from the rfe mutant lacked terminal glucose and was linear. These results are in agreement with recent findings on the biosynthesis of the 09 antigen. They suggest a cotransfer of glucose with the 09-specific mannan to a 'pre-core' lacking terminal glucose, as the assembly (translocation) step in the 09 antigen synthesis. Thus it is suggested that the initiation of O-chain synthesis (by the formation of an acceptor glucolipid ) and the termination of core synthesis are closely correlated. In conjunction with previous biochemical data, the analytical results presented here indicate a novel core synthesis.
利用成分分析、甲基化以及气相色谱/质谱分析法，对具有完整R1核心的大肠杆菌O9核心寡糖的外部（己糖）区域，以及一个源自R1的rfe突变体的核心寡糖外部区域的化学结构进行了分析。结果发现，与R1核心的分支状外部区域不同，rfe突变体核心的外部区域缺乏末端葡萄糖且呈线性。这些结果与最近关于O9抗原生物合成的研究结果一致。它们表明，在O9抗原合成的组装（易位）步骤中，葡萄糖与O9特异性甘露聚糖共同转移至缺乏末端葡萄糖的“前核心”。因此，有人提出O链合成的起始（通过形成受体糖脂）与核心合成的终止密切相关。结合先前的生化数据，此处给出的分析结果表明了一种新的核心合成方式。
