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rfe基因在大肠杆菌O7特异性脂多糖及其他含N-乙酰葡糖胺的O特异性多糖生物合成中的作用。

Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine.

作者信息

Alexander D C, Valvano M A

机构信息

Department of Microbiology and Immunology, University of Western Ontario, London, Canada.

出版信息

J Bacteriol. 1994 Nov;176(22):7079-84. doi: 10.1128/jb.176.22.7079-7084.1994.

DOI:10.1128/jb.176.22.7079-7084.1994
PMID:7525537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC197083/
Abstract

We report that rfe mutants of wild-type strains of Escherichia coli O7, O18, O75, and O111 did not express O-specific polysaccharide unless the rfe mutation was complemented by a cloned rfe gene supplied in a plasmid. The O polysaccharides in these strains are known to have N-acetylglucosamine (GlcNAc) in their O repeats. In addition, in vitro transferase assays with bacterial membranes from either the O7 wild-type strain or its isogenic rfe mutant showed that GlcNAc is the first carbohydrate added onto the lipid acceptor in the assembly of the O7 repeat and that this function is inhibited by tunicamycin. Our results indicate that the rfe gene product is a general requirement for the synthesis of O polysaccharides containing GlcNAc.

摘要

我们报告称，大肠杆菌O7、O18、O75和O111野生型菌株的rfe突变体不表达O特异性多糖，除非rfe突变通过质粒中提供的克隆rfe基因得到互补。已知这些菌株中的O多糖在其O重复序列中含有N - 乙酰葡糖胺（GlcNAc）。此外，用O7野生型菌株或其同基因rfe突变体的细菌膜进行的体外转移酶测定表明，GlcNAc是在O7重复序列组装过程中添加到脂质受体上的第一种碳水化合物，并且这种功能受到衣霉素的抑制。我们的结果表明，rfe基因产物是合成含GlcNAc的O多糖的普遍必需条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a126/197083/37e3d2ec529c/jbacter00040-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a126/197083/37e3d2ec529c/jbacter00040-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a126/197083/37e3d2ec529c/jbacter00040-0307-a.jpg

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