Puvion E, Viron A, Xu F X
Exp Cell Res. 1984 Jun;152(2):357-67. doi: 10.1016/0014-4827(84)90637-2.
Isolated rat liver cells were pulse-labelled with tritiated uridine and post-incubated in the presence of an excess of unlabelled uridine and of adenosine analog DRB (5-6-dichloro-1-beta-D-ribofuranosyl benzimidazole). Nuclear radioactivity was detected with high resolution autoradiography. A significant labelling of the interchromatin granules was revealed in these conditions. Pretreatments of cells with low doses of actinomycin D in order to preferentially inhibit ribosomal RNA (rRNA) synthesis prevented the labelling of the interchromatin granules during subsequent DRB treatments. These observations indicate that in DRB-treated cells, the interchromatin granules are sites of transfer or of accumulation of nucleolar RNA. Our results are discussed in connection with our knowledge of the action of DRB on RNA metabolism in mammalian cells and with recent data concerning the still enigmatic interchromatin granules which are present in the nuclei of most cells.
用氚标记的尿苷对分离的大鼠肝细胞进行脉冲标记，然后在过量未标记尿苷和腺苷类似物DRB（5-6-二氯-1-β-D-呋喃核糖基苯并咪唑）存在的情况下进行后续孵育。通过高分辨率放射自显影检测细胞核放射性。在这些条件下，染色质间颗粒出现了显著的标记。用低剂量放线菌素D预处理细胞以优先抑制核糖体RNA（rRNA）合成，可防止后续DRB处理期间染色质间颗粒的标记。这些观察结果表明，在DRB处理的细胞中，染色质间颗粒是核仁RNA转移或积累的部位。我们结合对DRB对哺乳动物细胞RNA代谢作用的了解以及有关大多数细胞核中存在的仍神秘的染色质间颗粒的最新数据来讨论我们的结果。
