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用碘化锌锇技术显示的组织细胞和树突状网状细胞。

Histiocytic and dendritic reticulum cells shown by a zinc iodide-osmium technique.

作者信息

Crocker J, Hopkins M

出版信息

J Clin Pathol. 1984 Jun;37(6):620-7. doi: 10.1136/jcp.37.6.620.

DOI:10.1136/jcp.37.6.620
PMID:6202719
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498834/
Abstract

Structures corresponding to histiocytic and dendritic reticulum cells have been shown in human tonsillar tissue, "reactive" lymph nodes and spleens by means of a zinc iodide-osmium technique. These cell types have been shown in various locations in these tissues using paraffin and resin embedded sections produced after fixation/staining of the tissue in zinc iodide-osmium. The quality of morphology attained by this procedure is much improved compared with the demonstration of the two cell types by means of alpha-naphthyl acetate esterase reactions performed on frozen sections. The zonal architecture of the lymphoid follicle is emphasised by this technique. In lymph nodes, sinus lining cells are also shown. Lymphoid cells, polymorphs, fibroblasts, and endothelial cells are negative with the zinc iodide-osmium method. In addition, interdigitating cells are not stained. The results of this procedure are compared with those with those of other methods for the demonstration of histiocytic and dendritic reticulum cells.

摘要

通过碘化锌-锇技术，在人类扁桃体组织、“反应性”淋巴结和脾脏中已显示出与组织细胞和树突状网状细胞相对应的结构。使用在碘化锌-锇中固定/染色后制作的石蜡和树脂包埋切片，已在这些组织的不同位置显示出这些细胞类型。与通过在冰冻切片上进行的α-萘乙酸酯酶反应来显示这两种细胞类型相比，该方法所获得的形态质量有了很大提高。该技术突出了淋巴滤泡的带状结构。在淋巴结中，也显示出窦衬细胞。淋巴细胞、多形核细胞、成纤维细胞和内皮细胞用碘化锌-锇法呈阴性。此外，交错突细胞不着色。将该方法的结果与用于显示组织细胞和树突状网状细胞的其他方法的结果进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/aff7c16b302f/jclinpath00177-0024-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/603d6ed5b4cb/jclinpath00177-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/227f02e302fd/jclinpath00177-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/b73dbed8c983/jclinpath00177-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/0fc4ea722908/jclinpath00177-0022-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/e1ddb64cdfb5/jclinpath00177-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/91662a66cd91/jclinpath00177-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/b31d733e0c80/jclinpath00177-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/0575acf599f9/jclinpath00177-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/fb9a9504111b/jclinpath00177-0024-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/aff7c16b302f/jclinpath00177-0024-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/603d6ed5b4cb/jclinpath00177-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/227f02e302fd/jclinpath00177-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/b73dbed8c983/jclinpath00177-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/0fc4ea722908/jclinpath00177-0022-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/e1ddb64cdfb5/jclinpath00177-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/91662a66cd91/jclinpath00177-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/b31d733e0c80/jclinpath00177-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/0575acf599f9/jclinpath00177-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/fb9a9504111b/jclinpath00177-0024-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b8/498834/aff7c16b302f/jclinpath00177-0024-d.jpg

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引用本文的文献

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2
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本文引用的文献

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Scanning and transmission electron microscopy on the epithelium of human palatine tonsils.人腭扁桃体上皮的扫描电镜和透射电镜观察
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The distribution of immunoglobulin and other plasma proteins in human reactive lymph nodes.免疫球蛋白及其他血浆蛋白在人类反应性淋巴结中的分布
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The form factor of alpha-naphthyl acetate esterase-positive cells in non-Hodgkin's lymphomas and reactive lymph nodes.非霍奇金淋巴瘤和反应性淋巴结中醋酸α-萘酯酶阳性细胞的形态因子。
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Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue.一种与人树突状网状细胞反应的单克隆抗体的制备及其在淋巴组织免疫组织学分析中的应用。
J Clin Pathol. 1983 Feb;36(2):167-75. doi: 10.1136/jcp.36.2.167.
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The enzyme histochemistry of lymphoid and non-lymphoid cells of the human palatine tonsil: a basis for the study of lymphomas.人类腭扁桃体淋巴样和非淋巴样细胞的酶组织化学：淋巴瘤研究的基础
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Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node.
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