Crivellato E, Mallardi F
Department of Medical and Morphological Research, University of Udine, Italy.
J Anat. 1997 Jan;190 ( Pt 1)(Pt 1):85-92. doi: 10.1046/j.1469-7580.1997.19010085.x.
The organisation of the stromal cell compartment in the mouse lymph node was studied by light and electron microscopy after tissue impregnation by the zinc iodide-osmium (ZIO) method. Fibroblastic reticular cells (FRCs) represented the main stromal cell population. These cells were located both in the cortical region and in the medulla and exhibited various configurations. In the cortex, FRCs were fusiform in shape and came into close proximity with the floor of the subcapsular sinus. In the medulla, the FRCs were shaped like irregular dendritic cells which formed a complex 3-dimensional network. The FRCs surrounded vascular structures such as capillaries and/or high endothelial venules; in these instances they were organised in a discontinuous sheath-like fashion around the vessel wall. By light and electron microscopy, FRCs have been observed to come in close spatial relationship with a number of cells in the lymph node, including sinus endothelial cells, the endothelium of high endothelial venules and capillaries, various types of lymphocytes, follicular dendritic cells and interdigitating cells. These microanatomical features are consistent with the proposal that FRCs may be involved in the communicative networks between the different lymph node compartments. In particular, the FRCs may be involved in the transport of molecules from the sinus compartment to the high endothelial venules or to the distinct cell populations in the lymphoid parenchyma.
采用碘化锌锇（ZIO）法对组织进行浸染后，利用光学显微镜和电子显微镜研究了小鼠淋巴结中基质细胞区室的组织情况。成纤维网状细胞（FRCs）是主要的基质细胞群体。这些细胞位于皮质区和髓质，呈现出多种形态。在皮质中，FRCs呈梭形，紧邻被膜下窦底部。在髓质中，FRCs形状如同不规则的树突状细胞，形成复杂的三维网络。FRCs围绕着诸如毛细血管和/或高内皮微静脉等血管结构；在这些情况下，它们以不连续的鞘状方式围绕血管壁排列。通过光学显微镜和电子显微镜观察到，FRCs与淋巴结中的许多细胞在空间上关系密切，包括窦内皮细胞、高内皮微静脉和毛细血管的内皮、各种类型的淋巴细胞、滤泡树突状细胞和交错突细胞。这些微观解剖学特征与FRCs可能参与不同淋巴结区室之间的通讯网络这一观点一致。特别是，FRCs可能参与分子从窦区室向高内皮微静脉或向淋巴实质中不同细胞群体的转运。
