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Method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens.

作者信息

Tavares de Castro J, San Miguel J F, Soler J, Catovsky D

出版信息

J Clin Pathol. 1984 Jun;37(6):628-32. doi: 10.1136/jcp.37.6.628.

DOI:10.1136/jcp.37.6.628
PMID:6202720
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498835/
Abstract

A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The morphology of the labelled cells is well preserved for analysis by light microscopy and the preparations obtained can be kept permanently. This method is useful for the analysis of mixed cell proliferations, particularly in leukaemias.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/89ae6329d197/jclinpath00177-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/d1e77bf714d3/jclinpath00177-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/e1781043aba0/jclinpath00177-0030-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/89ae6329d197/jclinpath00177-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/d1e77bf714d3/jclinpath00177-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/e1781043aba0/jclinpath00177-0030-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6064/498835/89ae6329d197/jclinpath00177-0031-a.jpg

相似文献

1
Method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens.
J Clin Pathol. 1984 Jun;37(6):628-32. doi: 10.1136/jcp.37.6.628.
2
Terminal deoxynucleotidyl transferase activity in acute leukemia: a study of 100 cases comparing an immunoperoxidase (PAP) vs immunofluorescent method.急性白血病中末端脱氧核苷酸转移酶活性：一项比较免疫过氧化物酶（PAP）与免疫荧光法的100例研究。
Am J Hematol. 1986 Jun;22(2):179-84. doi: 10.1002/ajh.2830220208.
3
Detection of terminal deoxynucleotidyl transferase (TdT)-positive leukemia cells by an immunoperoxidase staining.通过免疫过氧化物酶染色检测末端脱氧核苷酸转移酶（TdT）阳性白血病细胞。
Jpn J Med. 1984 May;23(2):114-7. doi: 10.2169/internalmedicine1962.23.114.
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Simultaneous evaluation for terminal deoxynucleotidyl transferase and myeloperoxidase in leukemia.白血病中末端脱氧核苷酸转移酶和髓过氧化物酶的同步评估。
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Immunoperoxidase determination of terminal deoxynucleotidyl transferase in acute leukemia using PAP and ABC methods: experience in 102 cases.采用PAP法和ABC法免疫过氧化物酶测定急性白血病中的末端脱氧核苷酸转移酶：102例经验
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Terminal deoxynucleotidyl transferase-positive acute myeloblastic leukemia.末端脱氧核苷酸转移酶阳性急性髓细胞白血病
Am J Hematol. 1981;10(3):251-8. doi: 10.1002/ajh.2830100304.

本文引用的文献

1
Phorbol ester-induced differentiation of a non-T, non-B leukemic cell line: model for human lymphoid progenitor cell development.佛波酯诱导的非T、非B白血病细胞系分化：人类淋巴祖细胞发育模型
J Immunol. 1982 Mar;128(3):1316-20.
2
Histochemical demonstration of terminal deoxynucleotidyl transferase in leukemia.
Blood. 1981 Oct;58(4):856-8.
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Cellular phenotypes of normal and leukemic hemopoietic cells determined by analysis with selected antibody combinations.通过使用选定的抗体组合进行分析来确定正常和白血病造血细胞的细胞表型。
Blood. 1980 Sep;56(3):430-41.
4
A differentiation antigen expressed selectively by a proportion of human blood cells: detection with a monoclonal antibody.一种在部分人类血细胞中选择性表达的分化抗原：用单克隆抗体进行检测。
Pathology. 1982 Jan;14(1):5-11. doi: 10.3109/00313028209069036.
5
A monoclonal antibody specific for immature human hemopoietic cells and T lineage cells.一种对未成熟人类造血细胞和T细胞谱系细胞具有特异性的单克隆抗体。
J Immunol. 1981 Dec;127(6):2269-74.
6
A monoclonal antibody to human acute lymphoblastic leukaemia antigen.一种针对人类急性淋巴细胞白血病抗原的单克隆抗体。
Nature. 1980 Feb 7;283(5747):583-5. doi: 10.1038/283583a0.
7
Monoclonal antibodies against antigens of the human platelet surface: preparation and properties.
Pathology. 1984 Jan;16(1):73-8. doi: 10.3109/00313028409067914.
8
Cytochemistry of normal lymphocyte subsets defined by monoclonal antibodies and immunocolloidal gold.用单克隆抗体和免疫胶体金定义的正常淋巴细胞亚群的细胞化学
Scand J Haematol. 1983 May;30(5):433-43. doi: 10.1111/j.1600-0609.1983.tb02531.x.
9
Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.用单克隆抗体进行光镜下白细胞细胞表面抗原检测的免疫金染色法：其在淋巴细胞亚群计数中的应用。
J Histochem Cytochem. 1983 Mar;31(3):376-81. doi: 10.1177/31.3.6186731.
10
The detection of intracellular antigens in human leucocytes by immunoperoxidase staining.通过免疫过氧化物酶染色检测人白细胞中的细胞内抗原。
Br J Haematol. 1975 Nov;31(3):361-70. doi: 10.1111/j.1365-2141.1975.tb00867.x.