Kelso A, Glasebrook A L
J Immunol. 1984 Jun;132(6):2924-31.
Sixty-four murine alloreactive, cytolytic and noncytolytic , T lymphocyte clones were tested for the production of interleukin 2 (IL 2), macrophage-activating factor (MAF), interferon (IFN), and colony-stimulating factor (CSF). Approximately 90% of both cytolytic and noncytolytic clones secreted MAF and IFN upon antigen or mitogen stimulation. IL 2, in contrast, was only released in detectable amounts by 50% of noncytolytic clones and 50% of a subclass of cytolytic clones in which the proliferation was independent of exogenous IL 2 ("antigen-driven" clones); IL 2-dependent cytolytic clones did not release measurable IL 2. CSF was secreted by approximately 90% of noncytolytic and IL 2-independent cytolytic clones and 40% of IL 2-dependent cytolytic clones. The analysis reported here revealed a strong quantitative correlation between the titers of MAF and IFN released by the clones, suggesting that these two assays may measure the same lymphokine. Although the other activities measured were not directly correlated, a broad association was noted between IL 2 secretion and the production of high titers of MAF, IFN, and CSF. Thus, noncytolytic and IL 2-independent cytolytic clones on average released significantly higher titers of these factors than IL 2-dependent cytolytic clones.
对64个鼠类同种异体反应性、溶细胞性和非溶细胞性T淋巴细胞克隆进行了白细胞介素2（IL-2）、巨噬细胞激活因子（MAF）、干扰素（IFN）和集落刺激因子（CSF）产生情况的检测。在抗原或丝裂原刺激下，约90%的溶细胞性和非溶细胞性克隆分泌MAF和IFN。相比之下，只有50%的非溶细胞性克隆以及50%的增殖不依赖外源性IL-2的溶细胞性克隆亚类（“抗原驱动”克隆）能释放可检测量的IL-2；依赖IL-2的溶细胞性克隆不释放可测量的IL-2。约90%的非溶细胞性和不依赖IL-2的溶细胞性克隆以及40%的依赖IL-2的溶细胞性克隆分泌CSF。此处报告的分析揭示了克隆释放的MAF和IFN效价之间存在很强的定量相关性，这表明这两种检测方法可能检测的是同一种淋巴因子。虽然所检测的其他活性没有直接相关性，但在IL-2分泌与高滴度MAF、IFN和CSF的产生之间发现了广泛的关联。因此，平均而言，非溶细胞性和不依赖IL-2的溶细胞性克隆释放的这些因子的效价比依赖IL-2的溶细胞性克隆显著更高。
