von Boehmer H, Kisielow P, Leiserson W, Haas W
J Immunol. 1984 Jul;133(1):59-64.
Approximately 30% of cytolytic Lyt-2+ clones from primed mice are able to proliferate autonomously, i.e., independent of IL 2 derived from Lyt-2- cells after antigenic stimulation. H-2K- or -D-restricted induction of Lyt-2+ cells to autonomous proliferation requires Ia+ stimulator cells. A strict correlation was observed between the ability of Lyt-2+ clones to proliferative autonomously and to induce DH. Eventually, the growth of all Lyt-2+ cytolytic clones becomes dependent on exogenous IL 2, and their ability to induce DH is lost. Small Lyt-2+ cells can also be induced in primary cultures by antigen or concanavalin A to proliferate in the absence of exogenous IL 2. The frequency of autonomously proliferating small Lyt-2+ cells is the same as that of small Lyt-2+ cells proliferating in the presence of exogenous IL 2. IL 2 derived from Lyt-2- cells can augment proliferation of Lyt-2+ cells, but is not obligatory.
来自致敏小鼠的约30%的细胞溶解型Lyt-2⁺克隆能够自主增殖，即抗原刺激后独立于源自Lyt-2⁻细胞的白细胞介素2（IL-2）进行增殖。H-2K⁻或-D限制的Lyt-2⁺细胞向自主增殖的诱导需要Ia⁺刺激细胞。观察到Lyt-2⁺克隆自主增殖和诱导迟发型超敏反应（DH）的能力之间存在严格的相关性。最终，所有Lyt-2⁺细胞溶解克隆的生长都变得依赖于外源性IL-2，并且它们诱导DH的能力丧失。在原代培养中，小Lyt-2⁺细胞也可被抗原或伴刀豆球蛋白A诱导在无外源性IL-2的情况下增殖。自主增殖的小Lyt-2⁺细胞的频率与在外源性IL-2存在下增殖的小Lyt-2⁺细胞的频率相同。源自Lyt-2⁻细胞的IL-2可增强Lyt-2⁺细胞的增殖，但并非必需。
