Balber A E, Amos D B, Artzt K
J Immunogenet. 1984 Apr;11(2):127-33. doi: 10.1111/j.1744-313x.1984.tb01047.x.
Monoclonal antibody 212.i.4.2 mediated complement-dependent lysis of spleen and lymph node cells carrying the tw1 , tw12 , tw71 , t6, tw73 , and tLub1 haplotypes, while cells from mice carrying 11 other t haplotypes were not lysed. The antibody also detected an epitope controlled by genes in the H-2Dd region of non-t mice. A molecule of 46,000 molecular weight was immunoprecipitated by 212.i.4.2 from detergent extracts of 125I-labelled spleen cells of +/ tw12 and B10.D2 mice. The H- 2dm2 mutation did not alter the expression of the epitope recognized by 212.i.4.2. However, the H- 2dm1 mutation decreased the reactivity of lymphoid cells with the antibody in cytotoxicity tests, and 212.i.4.2 immunoprecipitated little or no protein from extracts of B10.D2( R106 ) spleen cells which carry the H- 2dm1 mutation.
单克隆抗体212.i.4.2介导对携带tw1、tw12、tw71、t6、tw73和tLub1单倍型的脾细胞和淋巴结细胞的补体依赖性裂解，而携带其他11种t单倍型的小鼠细胞未被裂解。该抗体还检测到非t小鼠H-2Dd区域中基因控制的一个表位。通过212.i.4.2从+/tw12和B10.D2小鼠的125I标记脾细胞的去污剂提取物中免疫沉淀出分子量为46,000的分子。H-2dm2突变未改变212.i.4.2识别的表位的表达。然而，H-2dm1突变降低了淋巴细胞在细胞毒性试验中与该抗体的反应性，并且212.i.4.2从携带H-2dm1突变的B10.D2(R106)脾细胞提取物中免疫沉淀出很少或没有蛋白质。
