Hogarth P M, Walker I D, Rigby A J, McKenzie I F
Immunogenetics. 1983;18(6):617-24. doi: 10.1007/BF00345969.
The effect of the H-2dm1 mutation on Qa-m2 expression was examined. The mutant strain B10.D2-H-2dm1 showed a fourfold increase in Qa-m2 expression when compared with the parental strain B10.D2. Qa-m2 molecules immunoprecipitated from B10.D2-H-2dm1, C57BL/10, and B10.D2 spleen cells were identical by two-dimensional (2-D) gel electrophoresis [isoelectric focusing (IEF) followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE]). It is likely therefore that the increased Qa-m2 expression is not due to gross structural alterations of the Qa-m2 molecule; in the present study, alternative explanations are discussed.
研究了H-2dm1突变对Qa-m2表达的影响。与亲本菌株B10.D2相比，突变菌株B10.D2-H-2dm1的Qa-m2表达增加了四倍。通过二维（2-D）凝胶电泳[等电聚焦（IEF），然后是十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）]，从B10.D2-H-2dm1、C57BL/10和B10.D2脾细胞中免疫沉淀的Qa-m2分子是相同的。因此，Qa-m2表达增加可能不是由于Qa-m2分子的总体结构改变；在本研究中，讨论了其他解释。
