Neurath A R, Kent S B, Strick N
J Gen Virol. 1984 May;65 ( Pt 5):1009-14. doi: 10.1099/0022-1317-65-5-1009.
Peptides synthesized for potential application as antiviral vaccines have been mostly tested in the form of conjugates with carrier proteins. The possible use of several distinct synthetic vaccines in prophylaxis would be facilitated by the availability of fully synthetic immunogens. A synthetic peptide corresponding to residues 135 to 155 ( P135 -155) of hepatitis B surface antigen (HBsAg) failed to elicit in free form anti-peptide antibodies or anti-HBs. However, polymers of P135 -155 (prepared by linking to diaminoalkanes ) and synthetic conjugates prepared by binding P135 -155 to liposomes or polylysine were immunogenic. A poor correlation was observed between anti-peptide and anti-HBs responses elicited by these conjugates. Glutaraldehyde-fixed liposomes appeared to be the carriers of choice for inducing anti-HBs.
为作为抗病毒疫苗潜在应用而合成的肽大多以与载体蛋白的缀合物形式进行测试。完全合成免疫原的可得性将有助于几种不同合成疫苗在预防中的可能应用。对应于乙型肝炎表面抗原（HBsAg）135至155位残基（P135 - 155）的合成肽以游离形式未能引发抗肽抗体或抗HBs。然而，P135 - 155的聚合物（通过与二氨基烷烃连接制备）以及通过将P135 - 155与脂质体或聚赖氨酸结合制备的合成缀合物具有免疫原性。观察到这些缀合物引发的抗肽和抗HBs反应之间相关性较差。戊二醛固定的脂质体似乎是诱导抗HBs的首选载体。
