Coakham H B, Garson J A, Brownell B, Allan P M, Harper E I, Lane E B, Kemshead J T
Lancet. 1984 May 19;1(8386):1095-8. doi: 10.1016/s0140-6736(84)92508-x.
A panel of monoclonal antibodies was systematically applied to cerebrospinal fluid from 17 patients with suspected neoplastic meningitis and the results were compared with those obtained from routine cytological preparations. The antibody panel consisted of markers for neuroectodermal tissue ( UJ13A ), epithelial cytokeratin ( LE61 ), leucocytes ( 2D1 ), and neoplastic neuroblasts ( UJ181 .4). Additional antibodies were used to refine diagnosis when indicated. Cerebrospinal fluid samples from 12 patients with non-neoplastic conditions were used as controls. The use of monoclonal antibodies gave a positive diagnosis in 16/17 cases and the cells were accurately categorised as carcinoma (5/6 cases), neuroectodermal tumour (8/8 cases), and lymphoma (3/3 cases). In the 14 cases examined by routine cytology, malignant cells were reported in 10 cases and accurately categorised in only 3/14 cases. Immunocytological testing of cerebrospinal fluid with an antibody panel has greatly increased the accuracy with which malignant cells can be identified and categorised.
一组单克隆抗体被系统地应用于17例疑似肿瘤性脑膜炎患者的脑脊液，并将结果与常规细胞学涂片的结果进行比较。该抗体组包括神经外胚层组织标志物（UJ13A）、上皮细胞角蛋白（LE61）、白细胞（2D1）和肿瘤性成神经细胞（UJ181.4）。必要时使用其他抗体以完善诊断。12例非肿瘤性疾病患者的脑脊液样本用作对照。使用单克隆抗体在16/17例中做出了阳性诊断，细胞被准确分类为癌（5/6例）、神经外胚层肿瘤（8/8例）和淋巴瘤（3/3例）。在14例接受常规细胞学检查的病例中，10例报告有恶性细胞，其中仅3/14例被准确分类。用一组抗体对脑脊液进行免疫细胞学检测大大提高了识别和分类恶性细胞的准确性。
