Christensen K K, Christensen P, Duc G, Hitzig W H, Lindén V, Müller B, Seger R A
Pediatr Res. 1984 May;18(5):478-82. doi: 10.1203/00006450-198405000-00017.
The protective effect of four commercial human gammaglobulin batches (I-IV) in mice was studied using six different strains of group B streptococci (GBS): types Ia; Ib; II, R-protein negative (R-); II, R+; III, R-; and III, R+. Each mouse received 1.0 ml gammaglobulin and 0.5 ml bacteria, 10(6)-10(8) colony forming units (CFU). There was a close correlation between antibody levels measured by the use of radiolabeled protein A and the mouse-protective effect of the gamma-globulins. The mouse-protection tests demonstrated that batch I protected against GBS types Ia and III, R- at low concentration (65 mg/kg mouse weight), against type Ib at medium (260 mg/kg) and against type III, R+ at high concentration. Batch IV protected against types Ia and Ib, although the doses were four times higher than those in batch I, but did not protect against type III, R+. There was no mouse protection by any of the batches against type II. Antibody levels against Ibc and R, protein antigens, were substantially lower in batch IV. Because the results of these mouse-protection studies indicate the importance of such antibodies against protein antigens, batches I-III might be more useful for therapy of neonatal GBS-septicemia.
使用六种不同的B组链球菌（GBS）菌株：Ia型；Ib型；II型，R蛋白阴性（R-）；II型，R+；III型，R-；以及III型，R+，研究了四批市售人丙种球蛋白（I-IV）对小鼠的保护作用。每只小鼠接受1.0毫升丙种球蛋白和0.5毫升细菌，即10(6)-10(8)个菌落形成单位（CFU）。通过使用放射性标记蛋白A测量的抗体水平与丙种球蛋白对小鼠的保护作用之间存在密切相关性。小鼠保护试验表明，I批在低浓度（65毫克/千克小鼠体重）下可保护小鼠免受Ia型和III型，R-感染，在中等浓度（260毫克/千克）下可保护免受Ib型感染，在高浓度下可保护免受III型，R+感染。IV批可保护免受Ia型和Ib型感染，尽管剂量比I批高四倍，但不能保护免受III型，R+感染。没有一批能保护小鼠免受II型感染。IV批中针对Ibc和R蛋白抗原的抗体水平显著较低。由于这些小鼠保护研究的结果表明了此类针对蛋白抗原的抗体的重要性，I-III批可能对治疗新生儿GBS败血症更有用。
