Witte S
Ann N Y Acad Sci. 1983;416:426-40. doi: 10.1111/j.1749-6632.1983.tb35203.x.
The microcirculatory blood vessels of the exposed rat mesentery have been observed by incident fluorescence microscopy using various optical systems as well as image-intensifier devices and fast-scanning fluorometry techniques. As fluorescence-labeled proteins, albumin, gamma-globulin, fibrinogen, fibronectin, factor VIII-derived polypeptides, and antithrombin III have been injected intravenously. In some experiments two different-labeled proteins were administered consecutively. Whereas albumin and gamma-globulin did not accumulate at the inner surface of the microcirculatory blood vessels , fibrinogen and fibronectin showed a significant affinity to the vessels inner lining, mostly in venular vessels. Both substances accumulated at the same places of the microcirculatory endothelium. Blocking the fibrinolytic system of the animal increased the deposition of both substances. Factor VIII-derived polypeptides accumulated at the vessel inner surface in a different way. Antithrombin III did not show any affinities to the vascular wall. A receptor at the endothelial cell surface for both fibrinogen and fibronectin is postulated.
利用各种光学系统、图像增强装置和快速扫描荧光测定技术，通过入射荧光显微镜观察了暴露的大鼠肠系膜的微循环血管。作为荧光标记蛋白，白蛋白、γ-球蛋白、纤维蛋白原、纤连蛋白、凝血因子VIII衍生多肽和抗凝血酶III已通过静脉注射。在一些实验中，连续给予两种不同标记的蛋白。白蛋白和γ-球蛋白并未在微循环血管的内表面积聚，而纤维蛋白原和纤连蛋白对血管内膜显示出显著的亲和力，主要是在小静脉血管中。这两种物质都积聚在微循环内皮的相同部位。阻断动物的纤溶系统会增加这两种物质的沉积。凝血因子VIII衍生多肽以不同的方式积聚在血管内表面。抗凝血酶III对血管壁未显示出任何亲和力。推测在内皮细胞表面存在纤维蛋白原和纤连蛋白的受体。
