Root-Bernstein R S, Westall F C
Brain Res Bull. 1984 Apr;12(4):425-36. doi: 10.1016/0361-9230(84)90115-1.
We report the results of nuclear magnetic resonance spectroscopy studies of combinations of serotonin (5-hydroxytryptamine) with the tryptophan peptide sequence and similar peptides from myelin basic protein. The binding site appears to consist of the sequence Arg Phe Ser Trp. Similar serotonin binding sites were found to exist on LHRH (Tyr Ser Trp) and MSH-ACTH tetrapeptide (Phe Arg Trp). These binding sites are specific to serotonin as is demonstrated by lack of binding by dopamine, histamine, acetylcholine and a dozen other pharmacologically active amines and indoles. Drugs known to affect serotonin levels, e.g., fenfluramine and L-DOPA, bind weakly to these sites. Structural and functional similarities between the tryptophan peptide, LHRH, and MSH-ACTH with an ACTH-like peptide of human leukocyte interferon, with human and bovine serum albumin, hen ovalbumin, and with red pigment concentrating hormone suggest that the latter peptides may also contain similar serotonin binding sites. The elucidation of serotonin binding sites on these peptides and proteins has implications for understanding various aspects of cancer, autoimmunity, neurological disease, and peptide hormone control.
我们报告了血清素（5-羟色胺）与色氨酸肽序列以及髓鞘碱性蛋白中类似肽段组合的核磁共振光谱研究结果。结合位点似乎由精氨酸-苯丙氨酸-丝氨酸-色氨酸序列组成。在促黄体生成素释放激素（酪氨酸-丝氨酸-色氨酸）和促黑素-促肾上腺皮质激素四肽（苯丙氨酸-精氨酸-色氨酸）上也发现了类似的血清素结合位点。这些结合位点对血清素具有特异性，这一点通过多巴胺、组胺、乙酰胆碱以及其他十几种药理活性胺类和吲哚类物质不结合得以证明。已知会影响血清素水平的药物，如芬氟拉明和左旋多巴，与这些位点的结合较弱。色氨酸肽、促黄体生成素释放激素和促黑素-促肾上腺皮质激素与人白细胞干扰素的一种促肾上腺皮质激素样肽、人血清白蛋白、牛血清白蛋白、鸡卵清蛋白以及红色素浓缩激素之间的结构和功能相似性表明，后几种肽段可能也含有类似的血清素结合位点。阐明这些肽段和蛋白质上的血清素结合位点对于理解癌症、自身免疫、神经疾病以及肽类激素调控的各个方面具有重要意义。
