从猪下丘脑分离、结构分析、生物学特性鉴定及合成β-[酪氨酸9]促黑素细胞激素-(9 - 18)十肽

Isolation, structure, biological characterization, and synthesis of beta-[Tyr9]melanotropin-(9-18) decapeptide from pig hypothalami.

作者信息

Schally A V, Chang R C, Huang W Y, Coy D H, Kastin A J, Redding T W

出版信息

Proc Natl Acad Sci U S A. 1980 Jul;77(7):3947-51. doi: 10.1073/pnas.77.7.3947.

DOI:10.1073/pnas.77.7.3947

PMID:6254004

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC349744/

Abstract

A decapeptide with the amino acid sequence H-Tyr-Phe-Arg-Trp-Gly-Ser-Pro-Pro-Lys-Asp-OH was isolated from acid extracts of porcine hypothalami, structurally and biologically characterized, and synthesized. Except for the NH2-terminal tyrosine, this decapeptide corresponds to the amino acid sequences 9-18 of porcine beta-melanotropin (beta-MSH) and 49-58 of porcine beta-lipotropin (beta-LPH); it also has a tetrapeptide sequence of amino acids (Phe-Arg-Trp-Gly) common to the 7-10 sequences in corticotropin (ACTH) and alpha-MSH. beta-MSH, beta-LPH, alphaMSH, and ACTH from various species all have a histidine residue in the position immediately preceding the common sequence, and the occurrence of a natural peptide with the tyrosine residue in the corresponding site has not been previously reported. This suggests that the beta-[Tyr9]MSH-(9-18) decapeptide might be a fragment of a still larger precursor (prohormone) possibly related to beta-LPH.

摘要

从猪下丘脑的酸提取物中分离出一种氨基酸序列为H-Tyr-Phe-Arg-Trp-Gly-Ser-Pro-Pro-Lys-Asp-OH的十肽，对其进行了结构和生物学特性鉴定并合成。除了氨基末端的酪氨酸外，该十肽对应于猪β-促黑素（β-MSH）的第9 - 18位氨基酸序列和猪β-促脂素（β-LPH）的第49 - 58位氨基酸序列；它还具有促肾上腺皮质激素（ACTH）和α-MSH中第7 - 10位序列共有的四肽氨基酸序列（Phe-Arg-Trp-Gly）。来自不同物种的β-MSH、β-LPH、α-MSH和ACTH在共同序列之前紧邻的位置都有一个组氨酸残基，而在相应位点具有酪氨酸残基的天然肽的出现此前尚未见报道。这表明β-[Tyr9]MSH-(9 - 18)十肽可能是一个可能与β-LPH相关的更大前体（激素原）的片段。