Tagawa M, Tokuhisa T, Ono K, Taniguchi M, Herzenberg L A, Herzenberg L A
Cell Immunol. 1984 Jul;86(2):327-36. doi: 10.1016/0008-8749(84)90387-3.
Sequential immunization with a carrier molecule and a new epitope (hapten) conjugated to the carrier (carrier/hapten-carrier immunization) induces specific suppression for IgG antibody production to the new epitope (hapten) on the carrier. Once induced, this "epitope-specific" suppression persists and specifically suppresses subsequent in vivo IgG antibody responses to the hapten presented on the same or on an unrelated carrier molecule. In vitro studies presented here characterize the surface markers and specificity of suppressor T cells generated in carrier/hapten-carrier-immunized animals. Thus we show (1) that spleen cells from these donors suppress in vitro IgG anti-hapten antibody production by cocultured hapten-primed spleen cells; (2) that some but not all of the suppressor cells carry surface Lyt-2; (3) that at least some of the suppressor cells have receptors for the inducing hapten (DNP); and (4) that, unlike the suppression obtained in vivo, the in vitro suppression extends to IgG responses to unrelated carrier protein epitopes presented in association with the inducing hapten.
用载体分子和与载体偶联的新表位（半抗原）进行序贯免疫（载体/半抗原 - 载体免疫）可诱导对载体上的新表位（半抗原）产生IgG抗体的特异性抑制。一旦诱导产生，这种“表位特异性”抑制会持续存在，并特异性抑制随后体内针对相同或无关载体分子上呈现的半抗原的IgG抗体反应。本文所展示的体外研究表征了在载体/半抗原 - 载体免疫动物中产生的抑制性T细胞的表面标志物和特异性。因此，我们发现：（1）来自这些供体的脾细胞通过与共培养的经半抗原致敏的脾细胞共培养，在体外抑制IgG抗半抗原抗体的产生；（2）部分而非全部抑制性细胞携带表面Lyt - 2；（3）至少部分抑制性细胞具有针对诱导性半抗原（二硝基苯）的受体；（4）与体内获得的抑制不同，体外抑制扩展至对与诱导性半抗原联合呈现的无关载体蛋白表位的IgG反应。
