Sheets obtained by radiation polymerization for enzyme immunoassay.
作者信息
Kumakura M, Kaetsu I
出版信息
Int J Appl Radiat Isot. 1984 Jun;35(6):471-4. doi: 10.1016/0020-708x(84)90166-2.
PMID:6203845
Abstract
Immobilized anti-alpha-fetoprotein sheets, which were attached to sticks, for enzyme immunoassay of alpha-fetoprotein were prepared by radiation polymerization of hydrophilic monomers. The relationship between the preparation conditions and the activity of the sheets was studied. The activity varied with monomer concentration, hydrophilicity of polymer matrix, the amount of coating solution, and antibody concentration. The sheets obtained at relatively low monomer and antibody concentrations appeared to give a high activity. It was found that the sheets are applicable for the enzyme immunoassay of alpha-fetoprotein with high sensitivity.
摘要
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