通过辐射聚合固定抗体和酶标记抗体。
Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization.
作者信息
Kumakura M, Kaetsu I, Suzuki M, Adachi S
出版信息
Appl Biochem Biotechnol. 1983 Apr;8(2):87-96. doi: 10.1007/BF02778089.
Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization at low temperatures was studied. The antibody activity of antibody was not affected by irradiation at an irradiation dose of below 8 MR and low temperatures. Immobilization of peroxidase-labeled anti-rabbit IgG goat IgG, anti-peroxidase, peroxidase, and anti-alpha-fetoprotein was carried out with hydrophilic and hydrophobic monomers. The activity of the immobilized enzyme-labeled antibody membranes varied with the thickness of the membranes and increased with decreasing membrane thickness. The activity of the immobilized antibody particles was varied by particle size. Immobilized anti-alpha-fetoprotein particles and membranes can be used for the assay of alpha-fetoprotein by the antigen-antibody reaction, such as a solid-phase sandwich method with high sensitivity.
研究了在低温下通过辐射聚合固定抗体和酶标记抗体的方法。在低于8MR的辐照剂量和低温下，抗体的活性不受辐照影响。使用亲水性和疏水性单体固定过氧化物酶标记的抗兔IgG山羊IgG、抗过氧化物酶、过氧化物酶和抗甲胎蛋白。固定化酶标记抗体膜的活性随膜厚度而变化，且随膜厚度减小而增加。固定化抗体颗粒的活性因颗粒大小而异。固定化抗甲胎蛋白颗粒和膜可通过抗原-抗体反应用于甲胎蛋白的测定，如高灵敏度的固相夹心法。
Zotero 插件