日本根瘤菌类菌体对代谢物的主动吸收与被动吸收的评估。
Evaluation of active versus passive uptake of metabolites by Rhizobium japonicum bacteroids.
作者信息
Reibach P H, Streeter J G
出版信息
J Bacteriol. 1984 Jul;159(1):47-52. doi: 10.1128/jb.159.1.47-52.1984.
Abstract
Rhizobium japonicum bacteroids were isolated anaerobically from soybean [Glycine max (L.) Merr] nodules. The bacteroids, which were capable of acetylene reduction and respiration, were used to study the uptake of metabolites by a method which permits correction for nonspecific adsorption of metabolites and estimation of total cell volume. These determinations permit active uptake to be assessed from metabolite accumulation against a concentration gradient. Succinate, malate, alpha-ketoglutarate, and glutamate were absorbed via an active mechanism. Plots of 1/V versus 1/[S] for succinate and malate indicated the presence of two uptake components: a saturable and presumably active or carrier-mediated component and a nonsaturable and presumably passive component. The uptake of glucose, malonate, D-pinitol, myo-inositol, and glucose 6-phosphate was slow and not active.
摘要
从大豆[Glycine max (L.) Merr]根瘤中厌氧分离出日本根瘤菌类菌体。这些能够进行乙炔还原和呼吸作用的类菌体，被用于通过一种方法研究代谢物的摄取，该方法允许校正代谢物的非特异性吸附并估计总细胞体积。这些测定使得能够根据代谢物逆浓度梯度的积累来评估主动摄取。琥珀酸、苹果酸、α-酮戊二酸和谷氨酸通过主动机制被吸收。琥珀酸和苹果酸的1/V对1/[S]的图表明存在两种摄取成分：一种可饱和的、可能是主动的或载体介导的成分，以及一种不饱和的、可能是被动的成分。葡萄糖、丙二酸、D-松醇、肌醇和6-磷酸葡萄糖的摄取缓慢且无活性。
相似文献
1
Evaluation of active versus passive uptake of metabolites by Rhizobium japonicum bacteroids.日本根瘤菌类菌体对代谢物的主动吸收与被动吸收的评估。
J Bacteriol. 1984 Jul;159(1):47-52. doi: 10.1128/jb.159.1.47-52.1984.
2
Involvement of glutamate in the respiratory metabolism of Bradyrhizobium japonicum bacteroids.谷氨酸在日本慢生根瘤菌类菌体呼吸代谢中的作用。
J Bacteriol. 1987 Feb;169(2):495-9. doi: 10.1128/jb.169.2.495-499.1987.
3
Periplasmic metabolism of glutamate and aspartate by intact Bradyrhizobium japonicum bacteroids.完整的日本慢生根瘤菌类菌体对谷氨酸和天冬氨酸的周质代谢
Biochim Biophys Acta. 1990 Sep 14;1035(3):257-65. doi: 10.1016/0304-4165(90)90087-d.
4
Discrimination of Rhizobium japonicum, Rhizobium lupini, Rhizobium trifolii, Rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate.通过对2-酮戊二酸、谷氨酸和磷酸盐的摄取来鉴别日本根瘤菌、羽扇豆根瘤菌、三叶草根瘤菌、豌豆根瘤菌及类菌体。
Arch Microbiol. 1976 Apr 1;107(3):257-62. doi: 10.1007/BF00425336.
5
Symbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum.豌豆根瘤菌C4-二羧酸转运突变体的共生特性
J Bacteriol. 1983 Jun;154(3):1403-13. doi: 10.1128/jb.154.3.1403-1413.1983.
6
[Glucose metabolism in isolated bacteroids of lupine nodules].[羽扇豆根瘤中分离类菌体的葡萄糖代谢]
Biokhimiia. 1980 Dec;45(12):2139-45.
7
Respiration supported nitrogenase activity of isolated Rhizobium meliloti bacteroids.呼吸作用支持分离出的苜蓿根瘤菌类菌体的固氮酶活性。
J Cell Biochem. 1988 Sep;38(1):35-49. doi: 10.1002/jcb.240380105.
8
Mechanism of regulation of glucose transport in Rhizobium leguminosarum.豆科根瘤菌中葡萄糖转运的调控机制。
J Bacteriol. 1982 Mar;149(3):872-9. doi: 10.1128/jb.149.3.872-879.1982.
9
Ammonia assimilation by rhizobium cultures and bacteroids.根瘤菌培养物和类菌体对氨的同化作用。
J Gen Microbiol. 1975 Jan;86(1):39-48. doi: 10.1099/00221287-86-1-39.
10
Acetylene reduction by transfilter suspension cultures of Rhizobium japonicum.日本根瘤菌跨膜悬浮培养物对乙炔的还原作用
Biochem Biophys Res Commun. 1975 Jun 16;64(4):1126-33. doi: 10.1016/0006-291x(75)90811-6.
引用本文的文献
1
Specificity and regulation of the dicarboxylate carrier on the peribacteroid membrane of soybean nodules.大豆根瘤菌周膜上二羧酸载体的特异性和调节。
Planta. 1990 Oct;182(3):437-44. doi: 10.1007/BF02411397.
2
An alternative succinate (2-oxoglutarate) transport system in Rhizobium tropici is induced in nodules of Phaseolus vulgaris.热带根瘤菌中的一种替代性琥珀酸（2-酮戊二酸）转运系统在菜豆根瘤中被诱导。
J Bacteriol. 2009 Aug;191(16):5057-67. doi: 10.1128/JB.00252-09. Epub 2009 Jun 5.
3
Uptake and Metabolism of Carbohydrates by Bradyrhizobium japonicum Bacteroids.根瘤菌菌体内碳水化合物的吸收和代谢。
Plant Physiol. 1987 Mar;83(3):535-40. doi: 10.1104/pp.83.3.535.
4
Decreased Exopolysaccharide Synthesis by Anaerobic and Symbiotic Cells of Bradyrhizobium japonicum.日本慢生根瘤菌厌氧及共生细胞胞外多糖合成减少
Plant Physiol. 1985 Oct;79(2):445-50. doi: 10.1104/pp.79.2.445.
5
Growth, Respiration, and Polypeptide Patterns of Bradyrhizobium sp. (Arachis) Strain 3G4b20 from Succinate- or Oxygen-Limited Continuous Cultures.琥珀酸或氧限制连续培养中慢生根瘤菌（花生）株 3G4b20 的生长、呼吸和多肽模式。
Appl Environ Microbiol. 1990 Apr;56(4):1025-32. doi: 10.1128/aem.56.4.1025-1032.1990.
6
Physiological Characterization of Dicarboxylate-Induced Pleomorphic Forms of Bradyrhizobium japonicum.二羧酸诱导的根瘤菌多形性的生理学特征。
Appl Environ Microbiol. 1989 Mar;55(3):666-71. doi: 10.1128/aem.55.3.666-671.1989.
7
Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti.来自苜蓿根瘤菌的一种新型天冬氨酸转氨酶编码基因的分离与鉴定
J Bacteriol. 1993 Jul;175(13):4186-96. doi: 10.1128/jb.175.13.4186-4196.1993.
8
Influence of amino acids on nitrogen fixation ability and growth of Azospirillum spp.氨基酸对固氮螺菌属细菌固氮能力和生长的影响
Appl Environ Microbiol. 1988 Jan;54(1):87-93. doi: 10.1128/aem.54.1.87-93.1988.
9
Involvement of glutamate in the respiratory metabolism of Bradyrhizobium japonicum bacteroids.谷氨酸在日本慢生根瘤菌类菌体呼吸代谢中的作用。
J Bacteriol. 1987 Feb;169(2):495-9. doi: 10.1128/jb.169.2.495-499.1987.
10
Mutants of Rhizobium meliloti defective in succinate metabolism.在琥珀酸代谢方面存在缺陷的苜蓿根瘤菌突变体。
J Bacteriol. 1988 Aug;170(8):3396-403. doi: 10.1128/jb.170.8.3396-3403.1988.
本文引用的文献
1
Metabolism of C-labeled photosynthate and distribution of enzymes of glucose metabolism in soybean nodules.大豆根瘤中碳标记光合产物的代谢及葡萄糖代谢酶的分布
Plant Physiol. 1983 Jul;72(3):634-40. doi: 10.1104/pp.72.3.634.
2
Potassium transport in corn roots : I. Resolution of kinetics into a saturable and linear component.玉米根中的钾转运：I. 将动力学分解为饱和成分和线性成分
Plant Physiol. 1982 Dec;70(6):1723-31. doi: 10.1104/pp.70.6.1723.
3
Utilization of aldehydes and alcohols by soybean bacteroids.大豆类菌体对醛类和醇类的利用
Plant Physiol. 1981 Aug;68(2):489-93. doi: 10.1104/pp.68.2.489.
4
Organic Acid Metabolism by Isolated Rhizobium japonicum Bacteroids.分离的日本根瘤菌类菌体的有机酸代谢
Plant Physiol. 1978 May;61(5):787-90. doi: 10.1104/pp.61.5.787.
5
C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens.三叶草中华根瘤菌 C(4)-二羧酸转运突变体在白车轴草上形成无效根瘤。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4284-8. doi: 10.1073/pnas.78.7.4284.
6
Respiratory Enzyme Systems in Symbiotic Nitrogen Fixation: III. The Respiration of Rhizobium from Legume Nodules and Laboratory Cultures.共生固氮中的呼吸酶系统：III. 豆科植物根瘤和实验室培养物中根瘤菌的呼吸作用。
J Bacteriol. 1940 Feb;39(2):187-96. doi: 10.1128/jb.39.2.187-196.1940.
7
The bacterial component of soybean root nodules; changes in respiratory activity, cell dry weight and nucleic acid content with increasing nodule age.大豆根瘤的细菌成分；随着根瘤年龄增加，呼吸活性、细胞干重和核酸含量的变化。
J Gen Microbiol. 1958 Oct;19(2):312-23. doi: 10.1099/00221287-19-2-312.
8
Succinate transport in Rhizobium leguminosarum.豆科根瘤菌中的琥珀酸转运
J Bacteriol. 1981 Oct;148(1):193-202. doi: 10.1128/jb.148.1.193-202.1981.
9
Mechanism of regulation of glucose transport in Rhizobium leguminosarum.豆科根瘤菌中葡萄糖转运的调控机制。
J Bacteriol. 1982 Mar;149(3):872-9. doi: 10.1128/jb.149.3.872-879.1982.
10
Carbohydrate transport in bacteria.细菌中的碳水化合物运输
Microbiol Rev. 1980 Sep;44(3):385-418. doi: 10.1128/mr.44.3.385-418.1980.
Zotero 插件