一种从大量溶液中浓缩微克级蛋白质的高效回收方法。
A high recovery method for concentrating microgram quantities of protein from large volumes of solution.
作者信息
Mahuran D, Clements P, Carrella M, Strasberg P M
出版信息
Anal Biochem. 1983 Mar;129(2):513-6. doi: 10.1016/0003-2697(83)90585-7.
PMID:6846845
Abstract
A method is presented for the recovery of 40-80% of the protein from a 1 microgram/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure.
摘要
本文介绍了一种从1微克/毫升溶液中回收40%-80%蛋白质的方法。最终的蛋白质沉淀不含去污剂和其他离子化合物，因此可与任何变性溶液兼容。该方法不会影响蛋白质的一级结构，使得最终沉淀成为用于确定蛋白质结构的任何分析程序的理想样品。
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