Decuyper J, Piette J, Van de Vorst A
Arch Int Physiol Biochim. 1983 Dec;91(5):471-6. doi: 10.3109/13813458309067996.
Furocoumarin derivatives (FCD) are investigated in order to determine their ability to photosensitize the production of activated oxygen species. Using the method based on the specific singlet oxygen (1O2) oxydation of cholesterol, all FCD except bergaten appeared to be able to produce 1O2 with various efficiencies. EPR spin trapping experiments show that photoexcited FCD produce hydroxyl radicals as detected by the formation of a DMPO-OH signal which can be abolished when the photosensitization reaction is carried out in the presence of specific OH scavengers. Moreover, the photo-ejection of hydrated electron (e-) by FCD is demonstrated by the loss of paramagnetic absorption of nitroxide free radicals as e- trap.
为了确定呋喃香豆素衍生物（FCD）使活性氧物种产生光致敏作用的能力，对其展开了研究。采用基于胆固醇特异性单线态氧（1O2）氧化的方法，除补骨脂素外，所有FCD似乎都能够以不同效率产生1O2。电子顺磁共振（EPR）自旋捕集实验表明，光激发的FCD会产生羟基自由基，这可通过DMPO-OH信号的形成检测到，当在特定的OH清除剂存在下进行光致敏反应时，该信号会消失。此外，FCD对水合电子（e-）的光发射通过作为e-陷阱的氮氧化物自由基顺磁吸收的损失得以证明。
