Tanaka K, Mori W
Appl Pathol. 1983;1(1):2-9.
Luxol fast blue (LFB) was found to stain inclusion bodies in liver tissue which was considered to have been infected by hepatitis virus. There were two types of inclusion bodies, which were quite similar in morphology but showed somewhat different stainability by several dyes. One was confirmed to be hepatitis B surface antigen (HBsAg), but the other was definitely not, as far as can be determined by the best present-day immunological and histological techniques. The latter was found less commonly in the liver of unselected, consecutive autopsy cases (which included some cases of hepatitis), rather commonly in cirrhotic liver without any trace of hepatitis B virus infection, and with extremely high incidence in liver carrying HBsAg. Electron microscopic examination of the liver with inclusion bodies of this type disclosed unfamiliar structures which resembled virus-like particles at the same area as LFB positivity. The significance of the novel inclusion body revealed by LFB stain in the liver is not clear at present. However, it could be interpreted as a morphological manifestation of non-A non-B hepatitis virus infection, if there are two types of human hepatitis viruses, B and non-A non-B, which are very closely related intrinsically or produce some common product(s) in infected hepatic cells.
发现卢克斯奥尔坚牢蓝（LFB）可使被认为感染了肝炎病毒的肝组织中的包涵体染色。存在两种类型的包涵体，它们在形态上非常相似，但用几种染料染色时显示出略有不同的染色性。一种被确认为乙型肝炎表面抗原（HBsAg），但就目前最好的免疫学和组织学技术所能确定的而言，另一种肯定不是。在未经挑选的连续尸检病例（包括一些肝炎病例）的肝脏中较少发现后者，在无任何乙型肝炎病毒感染迹象的肝硬化肝脏中相当常见，而在携带HBsAg的肝脏中发生率极高。对具有这种类型包涵体的肝脏进行电子显微镜检查，发现在与LFB阳性相同的区域有类似病毒样颗粒的不熟悉结构。目前，LFB染色在肝脏中显示的新型包涵体的意义尚不清楚。然而，如果存在两种人类肝炎病毒，即B型和非甲非乙型，它们在本质上密切相关或在受感染的肝细胞中产生一些共同产物，那么它可以被解释为非甲非乙型肝炎病毒感染的形态学表现。
