Lehner T, Jones T
Clin Exp Immunol. 1984 Jun;56(3):683-93.
The role of MHC class II antigens was investigated in the process of antigen binding by T8+ cells and monocytes (Mo) and in the functions of helper factor (HF) and suppressor factor (SF). Monoclonal antibodies (MoAbs) to HLA-DR, DC and SB determinants were used in immunofluorescence, inhibition of antigen binding and affinity chromatography of HF and SF. Indirect immunofluorescence studies suggest that T lymphocytes from peripheral blood of healthy subjects have a small proportion of cells expressing HLA-DR, beta chain determinants (1.4-3.8%). These belong predominantly to the T8+ subset of cells (4.6-8.8%), with only a very small proportion in the T4+ cells (0.1-1.8%). However, DC1 on DRw6+ T cells and SB2,3 on any HLA typed cells were found in significantly greater proportion than the DR antigens in both T8+ and T4+ cells, though this was again greater on T8+ (30 and 25%) than T4+ (8.3 and 14.4%) cells. Although Mo had a greatly increased proportion of cells with DR-beta chain determinants (27-45%) than the T8+ cells, the converse was found with DC1 and SB2,3 determinants (13.9 and 11.4%). Inhibition of 125I-streptococcal antigen (SA) binding to T8+ cells and to Mo by MoAbs to the class II antigens showed that DR-beta chain monomorphic or polymorphic antibodies and DC1 antibodies inhibited binding to both cell types by 66-94%. However, MoAbs to DR-alpha chains or to the SB2,3 determinant failed to yield significant inhibition. Affinity chromatography studies of HF and SF revealed that the DR-beta chain monomorphic and DC1 antibodies bound HF and SF activities and that this was not found with the DR-beta chain polymorphic or SB2,3 antibodies. The results of inhibition of 125I-SA binding to T8+ cells and Mo, and absorption of HF and SF by affinity chromatography with MoAbs suggest four categories of recognition of human MHC class II antigenic determinants. (1) Class II determinants shared by the T8+ cells, Mo, HF and SF and recognized by MoAbs to monomorphic beta chains (DA6.231) and to DC1. (2) Class II determinants shared only by the SA binding T8+ cells and Mo and recognized by the MoAbs to a polymorphic beta chain (DA6.164) and to a monomorphic DR determinant (OK.Ial). (3) Class II determinants shared only by the HF and SF and recognized by the MoAbs to one of the alpha chains (TAL.1B5). (4) Class II determinants not detected on the two cells or the two T cell factors.
研究了MHC II类抗原在T8 +细胞和单核细胞（Mo）结合抗原过程中以及辅助因子（HF）和抑制因子（SF）功能中的作用。使用针对HLA - DR、DC和SB决定簇的单克隆抗体（MoAbs）进行免疫荧光、抗原结合抑制以及HF和SF的亲和层析研究。间接免疫荧光研究表明，健康受试者外周血中的T淋巴细胞有一小部分细胞表达HLA - DRβ链决定簇（1.4 - 3.8%）。这些细胞主要属于T8 +细胞亚群（4.6 - 8.8%），在T4 +细胞中所占比例极小（0.1 - 1.8%）。然而，发现DRw6 + T细胞上的DC1以及任何HLA分型细胞上的SB2,3在T8 +和T4 +细胞中的比例均显著高于DR抗原，不过在T8 +细胞上的比例（分别为30%和25%）再次高于T4 +细胞（分别为8.3%和14.4%）。尽管Mo中具有DRβ链决定簇的细胞比例（27 - 45%）比T8 +细胞大幅增加，但DC1和SB2,3决定簇的情况则相反（分别为13.9%和11.4%）。针对II类抗原的MoAbs抑制125I - 链球菌抗原（SA）与T8 +细胞和Mo的结合，结果显示DRβ链单态或多态抗体以及DC1抗体抑制两种细胞类型的结合达66 - 94%。然而，针对DRα链或SB2,3决定簇的MoAbs未能产生显著抑制作用。HF和SF的亲和层析研究表明，DRβ链单态和DC1抗体结合HF和SF活性，而DRβ链多态或SB2,3抗体则未发现这种情况。125I - SA与T8 +细胞和Mo结合的抑制结果以及用MoAbs进行亲和层析对HF和SF的吸附结果表明，人类MHC II类抗原决定簇存在四类识别情况。（1）T8 +细胞、Mo、HF和SF共有的II类决定簇，可被针对单态β链（DA6.231）和DC1的MoAbs识别。（2）仅SA结合的T8 +细胞和Mo共有的II类决定簇，可被针对多态β链（DA6.164）和单态DR决定簇（OK.Ial）的MoAbs识别。（3）仅HF和SF共有的II类决定簇，可被针对α链之一（TAL.1B5）的MoAbs识别。（4）在这两种细胞或两种T细胞因子上未检测到的II类决定簇。
