pMB1复制的控制：Rop对引物形成的抑制作用需要RNA1。
Control of pMB1 replication: inhibition of primer formation by Rop requires RNA1.
作者信息
Cesareni G, Cornelissen M, Lacatena R M, Castagnoli L
出版信息
EMBO J. 1984 Jun;3(6):1365-9. doi: 10.1002/j.1460-2075.1984.tb01978.x.
Abstract
The rop gene participates in the control of plasmid copy number by interfering with transcripts originating from the primer promoter. Here we show that this inhibition mechanism requires RNA1 in trans. Mutations in the RNA1 coding sequence that result in plasmids with altered incompatibility properties do not affect the ability of the molecule to participate in the Rop inhibition mechanism. Furthermore we show that the target of the Rop-RNA1 inhibitory mechanism is located, at least in part, after the 52nd nucleotide of the sequence encoding the primer transcript.
摘要
rop基因通过干扰源自引物启动子的转录本参与质粒拷贝数的控制。在此我们表明，这种抑制机制在反式作用中需要RNA1。RNA1编码序列中的突变导致质粒具有改变的不相容性特性，但并不影响该分子参与Rop抑制机制的能力。此外，我们表明Rop-RNA1抑制机制的靶标至少部分位于编码引物转录本的序列的第52个核苷酸之后。
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