Zhao Jiping, Hu Erling, Shang Sen, Wu Dai, Li Peng, Zhang Peng, Tan Dan, Lu Xiaoyun
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
Institute of Applied Electronics, China Academy of Engineering Physics, 64 Mianshan Road, Mianyang, 621900, China.
Biomed Opt Express. 2020 Jun 22;11(7):3890-3899. doi: 10.1364/BOE.392838. eCollection 2020 Jul 1.
In recent years, many studies have been conducted to investigate the non-thermal effects of THz radiation on different organisms, but further studies are needed to fully elucidate the effects, especially on the molecular level. In this study, we explored the effects of at 3.1 THz radiation on protein expression in ) using red fluorescent protein as a reporter molecule. After 8 hours of continuous THz irradiation of bacteria on LB (Luria-Bertani) solid plates at an average power of 33 mW/cm and 10 Hz pulse repetition frequency, we found that the plasmid copy number, protein expression and fluorescence intensity of bacteria from the irradiated area were 3.8-, 2.7-, and 3.3 times higher than in bacteria from the un-irradiated area, respectively. These findings suggest that plasmid replication changed significantly in bacteria exposed to 3.1 THz radiation, resulting in increased protein expression as evidenced by increased fluorescence intensity of the RFP reporter.
近年来，已经开展了许多研究来调查太赫兹辐射对不同生物体的非热效应，但仍需要进一步研究以充分阐明这些效应，尤其是在分子水平上的效应。在本研究中，我们以红色荧光蛋白作为报告分子，探索了3.1太赫兹辐射对（此处原文括号内内容缺失）蛋白质表达的影响。在平均功率为33毫瓦/平方厘米、脉冲重复频率为10赫兹的条件下，对LB（Luria-Bertani）固体平板上的细菌进行8小时连续太赫兹辐照后，我们发现，来自辐照区域的细菌的质粒拷贝数、蛋白质表达和荧光强度分别比未辐照区域的细菌高3.8倍、2.7倍和3.3倍。这些发现表明，暴露于3.1太赫兹辐射的细菌中质粒复制发生了显著变化，导致蛋白质表达增加，红色荧光蛋白报告分子荧光强度增加证明了这一点。
