Underwood P A
Mol Immunol. 1984 Jul;21(7):663-71. doi: 10.1016/0161-5890(84)90052-x.
Panels of monoclonal antibodies were raised to the haemagglutinin of strains of the Hong Kong subtype of influenza (H3N2), namely Hong Kong 1968, England 1972, Port Chalmers 1973, Victoria 1975 and Texas 1977. The probable binding sites of individual antibodies in each panel were determined by correlation of cross-reactivity with 16 heterologous strains from the same subtype, and amino acid differences between their haemagglutinin molecules. This led to an approximation of the "average repertoire" of the BALB/c mouse to influenza type A haemagglutinin. Comparative data of the cross-reactivity of whole mouse sera suggested that the monoclonal panels derived from spleen cells were a fair representation of circulating antibody. Variation in the cross-reactivities of individual sera approached that of individual monoclonal antibodies. The relevance of this finding to selection of new virus variants in the human population was discussed. Heterospecific antibodies were detected in some monoclonal panels. Specific amino acid changes which could be responsible for such activity were identified.
制备了针对流感香港亚型（H3N2）毒株血凝素的单克隆抗体组，这些毒株包括1968年香港株、1972年英格兰株、1973年查尔默斯港株、1975年维多利亚株和1977年得克萨斯株。通过将交叉反应性与来自同一亚型的16种异源毒株进行关联，以及分析它们血凝素分子之间的氨基酸差异，确定了每个单克隆抗体组中各个抗体可能的结合位点。这使得能够大致了解BALB/c小鼠对甲型流感血凝素的“平均抗体谱”。全鼠血清交叉反应性的比较数据表明，源自脾细胞的单克隆抗体组能较好地代表循环抗体。个体血清交叉反应性的变化与个体单克隆抗体的变化相近。讨论了这一发现与人群中新病毒变体选择的相关性。在一些单克隆抗体组中检测到了异种特异性抗体。确定了可能导致这种活性的特定氨基酸变化。
