Alessandrini C, Cavallotti C, De Rossi M, Fruschelli C, Gerli R, Mione M C, Sacchi G, Amenta F
Pharmacology. 1984;29(1):17-23. doi: 10.1159/000137987.
The localization of dopamine receptors within rabbit renal artery was studied using 3H-spiroperidol as a label for dopamine receptors and a histoautoradiographic technique. Preliminary radioreceptor binding studies showed that 3H-spiroperidol was bound to sections of renal artery in a manner consistent with the existence of dopamine receptors. In fact the binding was found to be saturable, stereospecific and of high affinity, with a Bmax approximately of 158.3 fmol/mg protein and a Kd of 13.5 nmol/l. The microscopic examination of sections processed for the histoautoradiographic demonstration of 3'-spiroperidol binding sites showed that the distribution of dopamine receptors in the renal artery was widespread. The highest concentration of dopamine receptors was found primarily in the smooth muscle cells of the media and then, in the following order, in endothelial cells of the intima and in fibrous and connective cells of the adventitia. The direct demonstration of dopamine receptors in the media of rabbit renal artery strongly supports the hypothesis that these receptors may be involved in the relaxation of the artery caused by infusion or application of dopamine.
利用³H-螺哌啶醇作为多巴胺受体的标记物，并采用组织放射自显影技术，对兔肾动脉内多巴胺受体的定位进行了研究。初步的放射受体结合研究表明，³H-螺哌啶醇与肾动脉切片的结合方式与多巴胺受体的存在一致。事实上，发现这种结合具有饱和性、立体特异性和高亲和力，Bmax约为158.3 fmol/mg蛋白，Kd为13.5 nmol/l。对经处理用于³'-螺哌啶醇结合位点组织放射自显影显示的切片进行显微镜检查发现，肾动脉中多巴胺受体的分布很广泛。多巴胺受体浓度最高的部位主要在中膜的平滑肌细胞，其次依次是内膜的内皮细胞以及外膜的纤维和结缔组织细胞。兔肾动脉中膜多巴胺受体的直接证明有力地支持了这样一种假说，即这些受体可能参与了多巴胺输注或应用所引起的动脉舒张。
